Targeting of SNAP-23 and SNAP-25 in polarized epithelial cells

SH Low, PA Roche, HA Anderson, SCD van Ijzendoorn, M Zhang, KE Mostov, T Weimbs*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

95 Citations (Scopus)

Abstract

SNAP-23 is the ubiquitously expressed homologue of the neuronal SNAP-25, which functions in synaptic vesicle fusion, We have investigated the subcellular localization of SNAP-23 in polarized epithelial cells, In hepatocyte-derived HepG2 cells and in Madin-Darby canine kidney (MDCK) cells, the majority of SNAP-23 was present at both the basolateral and apical plasma membrane domains with little intracellular localization, This suggests that SNAP-23 does not function in intracellular fusion events but rather as a general plasma membrane t-SNARE, Canine SNAP-23 is efficiently cleaved by the botulinum neurotoxin E, suggesting that it is the toxin-sensitive factor previously found to be involved in plasma membrane fusion in MDCK cells, The localization of SNAP-25 in transfected MDCK cells was studied for comparison and was found to be identical to SNAP-23 with the exception that SNAP-25 was transported to the primary cilia protruding from the apical plasma membrane, which suggests that subtle differences in the targeting signals of both proteins exist. In contrast to its behavior in neurons, the distribution of SNAP-25 in MDCK cells remained unaltered by treatment with dibutyryl cAMP or forskolin, which, however, caused an increased growth of the primary cilia, Finally, we found that SNAP-23/25 and syntaxin 1A, when coexpressed in MDCK cells, do not stably interact with each other but are independently targeted to the plasma membrane and lysosomes, respectively.

Original languageEnglish
Pages (from-to)3422-3430
Number of pages9
JournalThe Journal of Biological Chemistry
Volume273
Issue number6
Publication statusPublished - 6-Feb-1998

Keywords

  • CANINE KIDNEY-CELLS
  • SYNAPTOSOMAL-ASSOCIATED PROTEIN
  • NERVE GROWTH-FACTOR
  • POLYMERIC IMMUNOGLOBULIN RECEPTOR
  • SYNTAXIN-BINDING PROTEIN
  • PRIMARY CILIA
  • CLOSTRIDIAL NEUROTOXINS
  • BASOLATERAL TRANSPORT
  • NONNEURONAL TISSUES
  • NEURITE OUTGROWTH

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