Projects per year
Abstract
Many cell biological and biochemical mechanisms controlling the fundamental process of eukaryotic cell division have been identified; however, the temporal dynamics of biosynthetic processes during the cell division cycle are still elusive. Here, we show that key biosynthetic processes are temporally segregated along the cell cycle. Using budding yeast as a model and single-cell methods to dynamically measure metabolic activity, we observe two peaks in protein synthesis, in the G1 and S/G2/M phase, whereas lipid and polysaccharide synthesis peaks only once, during the S/G2/M phase. Integrating the inferred biosynthetic rates into a thermodynamic-stoichiometric metabolic model, we find that this temporal segregation in biosynthetic processes causes flux changes in primary metabolism, with an acceleration of glucose-uptake flux in G1 and phase-shifted oscillations of oxygen and carbon dioxide exchanges. Through experimental validation of the model predictions, we demonstrate that primary metabolism oscillates with cell-cycle periodicity to satisfy the changing demands of biosynthetic processes exhibiting unexpected dynamics during the cell cycle.
Original language | English |
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Pages (from-to) | 294-313 |
Number of pages | 20 |
Journal | Nature Metabolism |
Volume | 5 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1-Feb-2023 |
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Dive into the research topics of 'Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle'. Together they form a unique fingerprint.Datasets
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Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle
Takhaveev, V. (Creator), Ozsezen, S. (Creator), Smith, E. (Creator), Zylstra, A. (Creator), Chaillet, M. (Creator), Chen, H. (Creator), Papagiannakis, A. (Creator), Milias-Argeitis, A. (Creator) & Heinemann, M. (Creator), DataverseNL, 7-Jan-2023
DOI: 10.34894/xpyc7y
Dataset
Press/Media
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Waves of Macromolecule Production During the Cell Cycle
Heinemann, M. & Takhaveev, V.
01/06/2023 → 11/06/2023
2 items of Media coverage
Press/Media: Expert Comment › Popular
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Ritme van biosynthese is metabool bepaald
04/04/2023
1 item of Media coverage
Press/Media: Public Engagement Activities › Popular
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Nieuwe ontdekking over productie van DNA | Engineeringnet
03/03/2023
1 item of Media coverage
Press/Media: Expert Comment › Popular
Projects
- 1 Finished
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oLife Fellowship Programme
Roos, W. (PI), van der Tak, F. (PI), Zijlstra, W. (PI), Dobos, V. (Postdoc), Heinen, L. (Postdoc), Thangaratnarajah, C. (Postdoc), Hoekzema, M. (Postdoc), Blokhuis, A. (Postdoc), Mascotti, L. (Postdoc), Padin Santos, D. (Postdoc), Chopra, A. (Postdoc), Obermaier, S. (Postdoc), Driver, M. (Postdoc), Moreira Goulart, M. (Postdoc), Sasidharan, S. (Postdoc), Samar Mahapatra, S. (Postdoc), Zylstra, A. (Postdoc), Geiger, Y. (Postdoc), Llopis Lorente, A. (Postdoc), Aschmann, D. (Postdoc) & Kulala Vittala, S. (Postdoc)
01/04/2019 → 31/03/2024
Project: Research