Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

Dana I Colpa, Wen Zhou, Jan Pier Wempe, Jelmer Tamis, Marc C A Stuart, Janneke Krooneman, Gert-Jan W Euverink*

*Corresponding author for this work

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Abstract

Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L, and 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T.

Original languageEnglish
Article number19
Number of pages14
JournalAIMS Bioengineering
Volume7
Issue number1
DOIs
Publication statusPublished - 21-Feb-2020

Keywords

  • Thauera aminoaromatica MZ1T
  • biopolymers
  • polyhydroxyalkanoate (PHA)
  • polyhydroxybutyrate (PHB)
  • 16S-rDNA analysis
  • biodiversity
  • mixed microbial consortium

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