The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase

Bert Overduin; Saskia A. Hogenhout; Erik A. van der Biezen; Michel A. Haring; H. John J. Nijkamp; Jacques Hille

doi:10.1007/BF00276882

The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase

Bert Overduin, Saskia A. Hogenhout, Erik A. van der Biezen, Michel A. Haring, H. John J. Nijkamp, Jacques Hille

Groningen Biomolecular Sciences and Biotechnology Institute

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Abstract

The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (ACTase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus.

Original language	English
Number of pages	6
Journal	MGG Molecular %26 General Genetics
Volume	240
Issue number	1
DOIs	https://doi.org/10.1007/BF00276882
Publication status	Published - 1993

Keywords

RFLP linkage analysis
PCR
aspartate carbamoyltransferase
Asc locus
Tomato

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@article{a189b326a56f408aa633aebcdfa851ef,

title = "The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase",

abstract = "The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (ACTase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus.",

keywords = "RFLP linkage analysis, PCR, aspartate carbamoyltransferase, Asc locus, Tomato",

author = "Bert Overduin and Hogenhout, {Saskia A.} and Biezen, {Erik A. van der} and Haring, {Michel A.} and Nijkamp, {H. John J.} and Jacques Hille",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1993",

doi = "10.1007/BF00276882",

language = "English",

volume = "240",

journal = "MGG Molecular %26 General Genetics",

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TY - JOUR

T1 - The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase

AU - Overduin, Bert

AU - Hogenhout, Saskia A.

AU - Biezen, Erik A. van der

AU - Haring, Michel A.

AU - Nijkamp, H. John J.

AU - Hille, Jacques

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1993

Y1 - 1993

N2 - The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (ACTase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus.

AB - The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (ACTase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus.

KW - RFLP linkage analysis

KW - PCR

KW - aspartate carbamoyltransferase

KW - Asc locus

KW - Tomato

U2 - 10.1007/BF00276882

DO - 10.1007/BF00276882

M3 - Article

VL - 240

JO - MGG Molecular %26 General Genetics

JF - MGG Molecular %26 General Genetics

IS - 1

ER -

The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase

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