The Bacillus subtilis Minimal Genome Compendium

Stephan Michalik, Alexander Reder, Björn Richts, Patrick Faßhauer, Ulrike Mäder, Tiago Pedreira, Anja Poehlein, Auke J van Heel, Amanda Y van Tilburg, Josef Altenbuchner, Anika Klewing, Daniel R Reuß, Rolf Daniel, Fabian M Commichau, Oscar P Kuipers, Leendert W Hamoen, Uwe Völker*, Jörg Stülke*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

To better understand cellular life, it is essential to decipher the contribution of individual components and their interactions. Minimal genomes are an important tool to investigate these interactions. Here, we provide a database of 105 fully annotated genomes of a series of strains with sequential deletion steps of the industrially relevant model bacterium Bacillus subtilis starting with the laboratory wild type strain B. subtilis 168 and ending with B. subtilis PG38, which lacks approximately 40% of the original genome. The annotation is supported by sequencing of key intermediate strains as well as integration of literature knowledge for the annotation of the deletion scars and their potential effects. The strain compendium presented here represents a comprehensive genome library of the entire MiniBacillus project. This resource will facilitate the more effective application of the different strains in basic science as well as in biotechnology.

Original languageEnglish
Article numberacssynbio.1c00339
Pages (from-to)2767-2771
Number of pages5
JournalACS Synthetic Biology
Volume10
Issue number10
Early online date29-Sep-2021
DOIs
Publication statusPublished - Nov-2021

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