The bioconversion process of deoxypodophyllotoxin with Linum flavum cell cultures

A Koulman; A.C Beekman; N. Pras; Wim Quax

doi:10.1055/s-2003-42785

The bioconversion process of deoxypodophyllotoxin with Linum flavum cell cultures

A Koulman^*, A.C Beekman, N. Pras, Wim Quax

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7'-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of beta-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and beta-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as beta-peltatin glucoside

Original language	English
Pages (from-to)	739 - 744
Number of pages	6
Journal	Planta Medica
Volume	69
Issue number	8
DOIs	https://doi.org/10.1055/s-2003-42785
Publication status	Published - 2003

Keywords

Linum flavum
Linaceae
bioconversion
deoxypodophyllotoxin
lignans
podophyllotoxin
ANTHRISCUS-SYLVESTRIS
BIOSYNTHESIS

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10.1055/s-2003-42785

Cite this

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title = "The bioconversion process of deoxypodophyllotoxin with Linum flavum cell cultures",

abstract = "The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7'-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of beta-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and beta-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as beta-peltatin glucoside",

keywords = "Linum flavum, Linaceae, bioconversion, deoxypodophyllotoxin, lignans, podophyllotoxin, ANTHRISCUS-SYLVESTRIS, BIOSYNTHESIS",

author = "A Koulman and A.C Beekman and N. Pras and Wim Quax",

note = "Times Cited: 0 rticle nglish oulman, A niv Groningen, GUIDE, Dept Pharmaceut Biol, A Deusinglaan 1, NL-9713 AV Groningen, Netherlands ited References Count: 13 31AJ UDIGERSTR 14, D-70469 STUTTGART, GERMANY TUTTGART",

year = "2003",

doi = "10.1055/s-2003-42785",

language = "English",

volume = "69",

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journal = "Planta Medica",

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publisher = "GEORG THIEME VERLAG KG",

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TY - JOUR

T1 - The bioconversion process of deoxypodophyllotoxin with Linum flavum cell cultures

AU - Koulman, A

AU - Beekman, A.C

AU - Pras, N.

AU - Quax, Wim

N1 - Times Cited: 0 rticle nglish oulman, A niv Groningen, GUIDE, Dept Pharmaceut Biol, A Deusinglaan 1, NL-9713 AV Groningen, Netherlands ited References Count: 13 31AJ UDIGERSTR 14, D-70469 STUTTGART, GERMANY TUTTGART

PY - 2003

Y1 - 2003

N2 - The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7'-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of beta-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and beta-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as beta-peltatin glucoside

AB - The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7'-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of beta-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and beta-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as beta-peltatin glucoside

KW - Linum flavum

KW - Linaceae

KW - bioconversion

KW - deoxypodophyllotoxin

KW - lignans

KW - podophyllotoxin

KW - ANTHRISCUS-SYLVESTRIS

KW - BIOSYNTHESIS

U2 - 10.1055/s-2003-42785

DO - 10.1055/s-2003-42785

M3 - Article

VL - 69

SP - 739

EP - 744

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 8

ER -