The bioconversion process of deoxypodophyllotoxin with Linum flavum cell cultures

A Koulman*, A.C Beekman, N. Pras, Wim Quax

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7'-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of beta-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and beta-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as beta-peltatin glucoside
Original languageEnglish
Pages (from-to)739 - 744
Number of pages6
JournalPlanta Medica
Volume69
Issue number8
DOIs
Publication statusPublished - 2003

Keywords

  • Linum flavum
  • Linaceae
  • bioconversion
  • deoxypodophyllotoxin
  • lignans
  • podophyllotoxin
  • ANTHRISCUS-SYLVESTRIS
  • BIOSYNTHESIS

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