The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(L-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells

R. J. Kroeze, M. N. Helder, W. H. Roos, G. J. L. Wuite, R. A. Bank, T. H. Smit*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Bioabsorbable polymers are increasingly being used in tissue engineering strategies. Despite the knowledge that some sterilization techniques may affect the physical properties of these polymers, this aspect is often overlooked. We speculate that the type of sterilization method used may influence cellular responses by altering the surface characteristics. We cultured adipose stem cells on bioabsorbable poly(L-lactide-co-caprolactone) (PLCL) sheets, sterilized using either ethylene oxide (EO), argon glow discharge (aGD) or electron beam (e-beam). Significantly higher values for surface roughness in the order EO > aGD > e-beam and significant differences in contact angles (EO > e-beam > aGD) and surface energies (aGD > e-beam > EO) were observed. Increased cell attachment and proliferation rates were observed with lower contact angles. The alkaline phosphatase activity was significantly higher for the ethylene oxide sterilized PLCL sheet. In conclusion, the type of sterilization for bioabsorbable polymers should be considered in the design of new scaffolds, since it might affect, or can be used to enhance, the outcome of the tissue engineered construct. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)2060-2065
Number of pages6
JournalActa Biomaterialia
Volume6
Issue number6
DOIs
Publication statusPublished - Jun-2010

Keywords

  • Stem cell
  • Biodegradation
  • Sterilization
  • Bone tissue engineering
  • Polylactic acid
  • POLYMER-DEMIXED NANOTOPOGRAPHY
  • IN-VITRO
  • PLASMA TREATMENT
  • STROMAL CELLS
  • BONE
  • STERILIZATION
  • ADHESION
  • PROLIFERATION
  • WETTABILITY
  • GROWTH

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