Abstract
OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.
METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg(-1) bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg(-1) bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.
RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.
CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.
| Original language | English |
|---|---|
| Pages (from-to) | 591-601 |
| Number of pages | 11 |
| Journal | Oral diseases |
| Volume | 20 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Sept-2014 |
Keywords
- periodontitis
- pregnancy complications
- rat
- hypertension
- LOW-DOSE ENDOTOXIN
- FETAL-GROWTH RESTRICTION
- EXPERIMENTAL PREECLAMPSIA
- TROPHOBLAST RESPONSES
- PERIODONTAL PATHOGENS
- ESCHERICHIA-COLI
- TNF-ALPHA
- HYPERTENSION
- INFLAMMATION
- INFECTION