The Expression of NTAL and Its Protein Interactors Is Associated With Clinical Outcomes in Acute Myeloid Leukemia

Carolina Hassibe Thome, Germano Aguiar Ferreira, Diego Antonio Pereira-Martins, Guilherme Augusto dos Santos, Douglas R. Almeida-Silveira, Isabel Weinhauser, Gustavo Antonio de Souza, Roos Houtsma, Jan Jacob Schuringa, Eduardo M. Rego, Vitor M. Faca*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Non-T cell activation linker (NTAL) membrane protein depletion from lipid rafts by alkylphospholipids or downregulation by shRNA knockdown decreases cell viability through regulation of the Akt/PI3K pathway in mantle cell lymphoma and acute promyelocytic leukemia cells. Here, we confirmed that the knockdown of NTAL in acute myeloid leukemia (AML) cell lines was associated with decreased cell proliferation and survival. Similarly, a xenograft model using AML cells transduced with NTAL-shRNA and transplanted into immunodeficient mice led to a 1.8-fold decrease in tumor burden. Using immunoprecipitation, LC-MS/MS analysis, and label-free protein quantification, we identified interactors of NTAL in two AML cell lines. By evaluating the gene expression signatures of the NTAL protein interactors using the PREdiction of Clinical Outcomes from Genomic Profiles database, we found that 12 NTAL interactors could predict overall survival in AML, in at least two independent cohorts. In addition, patients with AML exhibiting a high expression of NTAL and its interactors were associated with a leukemic granulocyte-macrophage progenitor-like state. Taken together, our data provide evidence that NTAL and its protein interactors are relevant to AML cell proliferation and survival and represent potential therapeutic targets for granulocyte-macrophage progenitor-like leukemias.

Original languageEnglish
Article number100091
Number of pages20
JournalMolecular & Cellular Proteomics
Volume20
DOIs
Publication statusPublished - 2021

Keywords

  • TRANSMEMBRANE ADAPTER PROTEINS
  • CELL ACTIVATION
  • T-CELLS
  • GENE
  • LINKER
  • CD44
  • REGULATORS
  • CYTOSCAPE
  • MOLECULE
  • MODELS

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