The function of GORASPs in Golgi apparatus organization in vivo

Rianne Grond, Tineke Veenendaal, Juan M. Duran, Ishier Raote, Johan H. van Es, Sebastiaan Corstjens, Laura Delfgou, Benaissa El Haddouti, Vivek Malhotra, Catherine Rabouille

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    Abstract

    In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (G0RA5P2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.

    Original languageEnglish
    Article number202004191
    Number of pages14
    JournalJournal of Cell Biology
    Volume219
    Issue number9
    DOIs
    Publication statusPublished - 7-Sep-2020

    Keywords

    • GRASP PROTEIN
    • STACKING
    • FRAGMENTATION
    • SECRETION
    • RIBBON
    • CISTERNAE
    • PATHWAY
    • MITOSIS
    • GM130

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