The genetics of plant metabolism

Joost J.B. Keurentjes; Jingyuan Fu; C.H.  Ric de Vos; Arjen Lommen; Robert D. Hall; Raoul J. Bino; Linus H.W. van der Plas; Ritsert C. Jansen; Dick Vreugdenhil; Maarten Koornneef; RD Hall; RJ Bino; LHW van der Plas

doi:10.1038/ng1815

The genetics of plant metabolism

Joost J.B. Keurentjes, Jingyuan Fu, C.H. Ric de Vos, Arjen Lommen, Robert D. Hall, Raoul J. Bino, Linus H.W. van der Plas, Ritsert C. Jansen, Dick Vreugdenhil, Maarten Koornneef, RD Hall, RJ Bino, LHW van der Plas

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Abstract

Variation for metabolite composition and content is often observed in plants. However, it is poorly understood to what extent this variation has a genetic basis. Here, we describe the genetic analysis of natural variation in the metabolite composition in Arabidopsis thaliana. Instead of focusing on specific metabolites, we have applied empirical untargeted metabolomics using liquid chromatography–time of flight mass spectrometry (LC-QTOF MS). This uncovered many qualitative and quantitative differences in metabolite accumulation between A. thaliana accessions. Only 13.4% of the mass peaks were detected in all 14 accessions analyzed. Quantitative trait locus (QTL) analysis of more than 2,000 mass peaks, detected in a recombinant inbred line (RIL) population derived from the two most divergent accessions, enabled the identification of QTLs for about 75% of the mass signals. More than one-third of the signals were not detected in either parent, indicating the large potential for modification of metabolic composition through classical breeding.

Original language	English
Pages (from-to)	842 - 849
Number of pages	8
Journal	Nature Genetics
Volume	38
Issue number	7
DOIs	https://doi.org/10.1038/ng1815
Publication status	Published - 2006

Keywords

QUANTITATIVE TRAIT LOCI
INTEGRATIVE GENOMICS APPROACH
ARABIDOPSIS-THALIANA
SECONDARY METABOLISM
SHOOT DEVELOPMENT
EXPRESSION
DIVERSIFICATION
CONSTRUCTION
ACCUMULATION
METABOLOMICS

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@article{37036a841b334115b51b4229bb2f4fa0,

title = "The genetics of plant metabolism",

abstract = "Variation for metabolite composition and content is often observed in plants. However, it is poorly understood to what extent this variation has a genetic basis. Here, we describe the genetic analysis of natural variation in the metabolite composition in Arabidopsis thaliana. Instead of focusing on specific metabolites, we have applied empirical untargeted metabolomics using liquid chromatography–time of flight mass spectrometry (LC-QTOF MS). This uncovered many qualitative and quantitative differences in metabolite accumulation between A. thaliana accessions. Only 13.4% of the mass peaks were detected in all 14 accessions analyzed. Quantitative trait locus (QTL) analysis of more than 2,000 mass peaks, detected in a recombinant inbred line (RIL) population derived from the two most divergent accessions, enabled the identification of QTLs for about 75% of the mass signals. More than one-third of the signals were not detected in either parent, indicating the large potential for modification of metabolic composition through classical breeding.",

keywords = "QUANTITATIVE TRAIT LOCI, INTEGRATIVE GENOMICS APPROACH, ARABIDOPSIS-THALIANA, SECONDARY METABOLISM, SHOOT DEVELOPMENT, EXPRESSION, DIVERSIFICATION, CONSTRUCTION, ACCUMULATION, METABOLOMICS",

author = "Keurentjes, {Joost J.B.} and Jingyuan Fu and Vos, {C.H. Ric de} and Arjen Lommen and Hall, {Robert D.} and Bino, {Raoul J.} and Plas, {Linus H.W. van der} and Jansen, {Ritsert C.} and Dick Vreugdenhil and Maarten Koornneef and RD Hall and RJ Bino and {van der Plas}, LHW",

note = "Relation: http://www.rug.nl/ Rights: University of Groningen",

year = "2006",

doi = "10.1038/ng1815",

language = "English",

volume = "38",

pages = "842 -- 849",

journal = "Nature Genetics",

issn = "1061-4036",

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T1 - The genetics of plant metabolism

AU - Keurentjes, Joost J.B.

AU - Fu, Jingyuan

AU - Vos, C.H. Ric de

AU - Lommen, Arjen

AU - Hall, Robert D.

AU - Bino, Raoul J.

AU - Plas, Linus H.W. van der

AU - Jansen, Ritsert C.

AU - Vreugdenhil, Dick

AU - Koornneef, Maarten

AU - Hall, RD

AU - Bino, RJ

AU - van der Plas, LHW

N1 - Relation: http://www.rug.nl/ Rights: University of Groningen

PY - 2006

Y1 - 2006

N2 - Variation for metabolite composition and content is often observed in plants. However, it is poorly understood to what extent this variation has a genetic basis. Here, we describe the genetic analysis of natural variation in the metabolite composition in Arabidopsis thaliana. Instead of focusing on specific metabolites, we have applied empirical untargeted metabolomics using liquid chromatography–time of flight mass spectrometry (LC-QTOF MS). This uncovered many qualitative and quantitative differences in metabolite accumulation between A. thaliana accessions. Only 13.4% of the mass peaks were detected in all 14 accessions analyzed. Quantitative trait locus (QTL) analysis of more than 2,000 mass peaks, detected in a recombinant inbred line (RIL) population derived from the two most divergent accessions, enabled the identification of QTLs for about 75% of the mass signals. More than one-third of the signals were not detected in either parent, indicating the large potential for modification of metabolic composition through classical breeding.

AB - Variation for metabolite composition and content is often observed in plants. However, it is poorly understood to what extent this variation has a genetic basis. Here, we describe the genetic analysis of natural variation in the metabolite composition in Arabidopsis thaliana. Instead of focusing on specific metabolites, we have applied empirical untargeted metabolomics using liquid chromatography–time of flight mass spectrometry (LC-QTOF MS). This uncovered many qualitative and quantitative differences in metabolite accumulation between A. thaliana accessions. Only 13.4% of the mass peaks were detected in all 14 accessions analyzed. Quantitative trait locus (QTL) analysis of more than 2,000 mass peaks, detected in a recombinant inbred line (RIL) population derived from the two most divergent accessions, enabled the identification of QTLs for about 75% of the mass signals. More than one-third of the signals were not detected in either parent, indicating the large potential for modification of metabolic composition through classical breeding.

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KW - INTEGRATIVE GENOMICS APPROACH

KW - ARABIDOPSIS-THALIANA

KW - SECONDARY METABOLISM

KW - SHOOT DEVELOPMENT

KW - EXPRESSION

KW - DIVERSIFICATION

KW - CONSTRUCTION

KW - ACCUMULATION

KW - METABOLOMICS

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DO - 10.1038/ng1815

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VL - 38

SP - 842

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The genetics of plant metabolism

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