The glycocalyx and stereociliary interconnections of the vestibular sensory epithelia of the guinea pig - A freeze-fracture, low-voltage cryo-SEM, SEM and TEM study

WL Valk, MLYM Oei*, JM Segenhout, F Dijk, [No Value] Stokroos, FWJ Albers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

In this study freeze fracture, low-voltage cryo-SEM, SEM and TEM were used to characterise the glycocalyx and stereociliary interconnections in the hair cell bundle of the vestibular sensory epithelia of the guinea pig. The glycocalyx resembles a shell-like structure separately surrounding each stereocilium and kinocilium over its entire length. The lateral interciliary connections emerge from the glycocalyx layer, forming an extensive extracellular network maintaining the stereocilia as a bundle. These connections are morphologically similar to the glycocalyx, and grossly oriented in the same direction, indicative of a role in the mechanical transduction system. The matrix material around the glycocalyx also appears to be morphologically similar to the glycocalyx, suggesting the glycocalyx to be even more important in the mechanical transduction system. The tip-links are covered with a layer, which is a continuation of the glycocalyx covering the stereocilia. Copyright (C) 2002 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)242-246
Number of pages5
JournalOrl-Journal for oto-Rhino-Laryngology and its related specialties
Volume64
Issue number4
Publication statusPublished - 2002

Keywords

  • glycocalyx
  • stereocilia
  • tip-link
  • freeze fracture
  • cryo-scanning electron microscopy
  • guinea pig
  • DEEP-ETCH
  • HAIR

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