The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein that is required for macropexophagy

Iryna Monastyrska, Jan A K W Kiel, Arjen M Krikken, Janet A Komduur, Marten Veenhuis, Ida J van der Klei

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35 Citations (Scopus)

Abstract

We have isolated the Hansenula polymorpha ATG25 gene, which is required for glucose-induced selective peroxisome degradation by macropexophagy. ATG25 represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein colocalizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). In cells of a constructed ATG25 deletion strain (atg25) peroxisomes are constitutively degraded by nonselective microautophagy, a process that in WT H. polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells.
Original languageEnglish
Pages (from-to)92 - 100
Number of pages9
JournalAutophagy
Volume1
Issue number2
Publication statusPublished - 2005

Keywords

  • yeast
  • microautophagy
  • pexophagy
  • ATG 11
  • ATG25
  • pre-autophagosomal structure
  • YEAST HANSENULA-POLYMORPHA
  • VACUOLE TARGETING PATHWAY
  • SELECTIVE DEGRADATION
  • PEROXISOME DEGRADATION
  • PICHIA-PASTORIS
  • BIOGENESIS
  • AUTOPHAGY
  • PROLIFERATION
  • CYTOPLASM
  • ENZYME

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