THE INFLUENCE OF SURFACE-FREE ENERGY ON SUPRAGINGIVAL AND SUBGINGIVAL PLAQUE MICROBIOLOGY - AN IN-VIVO STUDY ON IMPLANTS

M QUIRYNEN*, HC VANDERMEI, CML BOLLEN, LH VANDENBOSSCHE, GI DOORNBUSCH, D VANSTEENBERGHE, HJ BUSSCHER

*Corresponding author for this work

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Abstract

THE INFLUENCE OF SURFACE FREE ENERGY on supra- and subgingival plaque microbiology was examined in 9 patients with functional fixed prostheses supported by endosseous titanium implants. Two abutments (trans-mucosal part of the 2 stage implant) were replaced by either a new titanium abutment or a fluor-ethylene-propylene (FEP) coated abutment per subject. After 3 months of habitual oral hygiene, plaque samples were taken. Supragingival plaque was examined by means of differential phase-contrast microscopy, whereas for the subgingivial plaque additional analyses (DNA probes analysis, culturing) were performed. The subgingival samples were taken by paper-points and by scraping of the subgingival abutment surface. Differential phase-contrast microscopy showed a significant difference in plaque composition, especially when supragingival plaque was considered (P = 0.05). FEP coated abutments frequently harbored more coccoid microorganisms, whereas spirochetes or motile organisms were only detected around titanium abutments. Subgingivally, the number of colony forming units (CFU) in paper-points was comparable for both types of abutments. If the to-the-abutment-adhering plaque was considered, the number of CFU was 5 times higher on the titanium abutments than on the FEP coated abutments. However, this difference did not reach a statistical level of significance (P = 0.38). The DNA probe analysis of the subgingival plaque collected with paper-points showed a slightly higher frequency and concentration of perio-pathogens around the titanium abutments. However, the inter-substratum differences were smaller than the inter-subject differences. The latter seemed to be related to patient's dental status. These results indicate that lowering the surface-free energy of intra-oral hard surfaces results in a reduction in bacterial colonization and in a retardation in its maturation in the supragingival area. For the subgingival area, the influence of this surface characteristic is not as obvious.

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalJournal of Periodontology
Volume65
Issue number2
Publication statusPublished - Feb-1994

Keywords

  • DENTAL PLAQUE MICROBIOLOGY
  • DENTAL IMPLANTS, TITANIUM
  • SURFACE PROPERTIES
  • FLUORO-ETHYLENE-PROPYLENE
  • BACTERIAL ADHESION
  • STREPTOCOCCUS-SANGUIS
  • ORAL STREPTOCOCCI
  • SOLIDS
  • INVIVO
  • SULCUS
  • SITES
  • FLORA

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