The mitochondrial contact site complex, a determinant of mitochondrial architecture

Max Harner, Christian Körner, Dirk Walther, Dejana Mokranjac, Johannes Kaesmacher, Ulrich Welsch, Janice Griffith, Matthias Mann, Fulvio Reggiori, Walter Neupert, Fulvio Reggiori

Research output: Contribution to journalArticleAcademicpeer-review

330 Citations (Scopus)


Mitochondria are organelles with a complex architecture. They are bounded by an envelope consisting of the outer membrane and the inner boundary membrane (IBM). Narrow crista junctions (CJs) link the IBM to the cristae. OMs and IBMs are firmly connected by contact sites (CS). The molecular nature of the CS remained unknown. Using quantitative high-resolution mass spectrometry we identified a novel complex, the mitochondrial contact site (MICOS) complex, formed by a set of mitochondrial membrane proteins that is essential for the formation of CS. MICOS is preferentially located at the CJs. Upon loss of one of the MICOS subunits, CJs disappear completely or are impaired, showing that CJs require the presence of CS to form a superstructure that links the IBM to the cristae. Loss of MICOS subunits results in loss of respiratory competence and altered inheritance of mitochondrial DNA.

Original languageEnglish
Pages (from-to)4356-4370
Number of pages15
JournalThe EMBO Journal
Issue number21
Publication statusPublished - 2-Nov-2011
Externally publishedYes


  • Binding Sites
  • DNA, Mitochondrial
  • Intracellular Membranes
  • Membrane Proteins
  • Microscopy, Electron
  • Mitochondria
  • Mitochondrial Proteins
  • Models, Biological
  • Multiprotein Complexes
  • Organisms, Genetically Modified
  • Protein Binding
  • Protein Transport
  • Saccharomyces cerevisiae

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