The mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione

Lee M Booty, Martin S King, Chancievan Thangaratnarajah, Homa Majd, Andrew M James, Edmund R S Kunji, Michael P Murphy

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Glutathione carries out vital protective roles within mitochondria, but is synthesised in the cytosol. Previous studies have suggested that the mitochondrial dicarboxylate and 2-oxoglutarate carriers were responsible for glutathione uptake. We set out to characterise the putative glutathione transport by using fused membrane vesicles of Lactococcus lactis overexpressing the dicarboxylate and 2-oxoglutarate carriers. Although transport of the canonical substrates could be measured readily, an excess of glutathione did not compete for substrate uptake nor could transport of glutathione be measured directly. Thus these mitochondrial carriers do not transport glutathione and the identity of the mitochondrial glutathione transporter remains unknown.

Original languageEnglish
Pages (from-to)621-8
Number of pages8
JournalFEBS Letters
Issue number5
Publication statusPublished - 27-Feb-2015
Externally publishedYes


  • Dicarboxylic Acid Transporters/genetics
  • Glutathione/metabolism
  • Immunoblotting
  • Ketoglutaric Acids/metabolism
  • Lactococcus lactis/metabolism
  • Membrane Transport Proteins/metabolism
  • Mitochondria/metabolism
  • Polymerase Chain Reaction
  • Reactive Oxygen Species/metabolism

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