The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64

Rinze F. Neuteboom; Evert Verbraak; Annet F. Wierenga-Wolf; Jane S. A. Voerman; Marjan van Meurs; Sigrid M. Swagemakers; Peter J. van de Spek; Eric A. P. Steegers; Christianne J. M. de Groot; Jon D. Laman; Rogier Q. Hintzen

doi:10.1177/1352458510389628

The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64

Rinze F. Neuteboom, Evert Verbraak, Annet F. Wierenga-Wolf, Jane S. A. Voerman, Marjan van Meurs, Sigrid M. Swagemakers, Peter J. van de Spek, Eric A. P. Steegers, Christianne J. M. de Groot, Jon D. Laman, Rogier Q. Hintzen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

15 Downloads (Pure)

Abstract

Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration.

Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes.

Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy.

Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls.

Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions.

Original language	English
Pages (from-to)	389-396
Number of pages	8
Journal	Multiple sclerosis journal
Volume	17
Issue number	4
DOIs	https://doi.org/10.1177/1352458510389628
Publication status	Published - Apr-2011
Externally published	Yes

Keywords

autoimmunity
CD64
immune tolerance
innate immunity
multiple sclerosis
pregnancy
BLOOD MONONUCLEAR-CELLS
T-BET
IMMUNOGLOBULIN-G
HORMONE ESTRIOL
GAMMA
DISEASE
PSTAT1
PREECLAMPSIA
ACTIVATION
INFECTION

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The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64Final publisher's version, 167 KBLicence: Taverne

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Neuteboom, R. F., Verbraak, E., Wierenga-Wolf, A. F., Voerman, J. S. A., van Meurs, M., Swagemakers, S. M., de Spek, P. J. V., Steegers, E. A. P., de Groot, C. J. M., Laman, J. D., & Hintzen, R. Q. (2011). The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64. Multiple sclerosis journal, 17(4), 389-396. https://doi.org/10.1177/1352458510389628

@article{a5873f41e9c24afeaba90630f5960ac0,

title = "The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64",

abstract = "Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration.Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes.Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy.Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls.Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions.",

keywords = "autoimmunity, CD64, immune tolerance, innate immunity, multiple sclerosis, pregnancy, BLOOD MONONUCLEAR-CELLS, T-BET, IMMUNOGLOBULIN-G, HORMONE ESTRIOL, GAMMA, DISEASE, PSTAT1, PREECLAMPSIA, ACTIVATION, INFECTION",

author = "Neuteboom, \{Rinze F.\} and Evert Verbraak and Wierenga-Wolf, \{Annet F.\} and Voerman, \{Jane S. A.\} and \{van Meurs\}, Marjan and Swagemakers, \{Sigrid M.\} and \{de Spek\}, \{Peter J. van\} and Steegers, \{Eric A. P.\} and \{de Groot\}, \{Christianne J. M.\} and Laman, \{Jon D.\} and Hintzen, \{Rogier Q.\}",

year = "2011",

month = apr,

doi = "10.1177/1352458510389628",

language = "English",

volume = "17",

pages = "389--396",

journal = "Multiple sclerosis journal",

issn = "1352-4585",

publisher = "SAGE Publications Inc.",

number = "4",

}

Neuteboom, RF, Verbraak, E, Wierenga-Wolf, AF, Voerman, JSA, van Meurs, M, Swagemakers, SM, de Spek, PJV, Steegers, EAP, de Groot, CJM, Laman, JD & Hintzen, RQ 2011, 'The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64', Multiple sclerosis journal, vol. 17, no. 4, pp. 389-396. https://doi.org/10.1177/1352458510389628

TY - JOUR

T1 - The monocyte transcriptome during pregnancy in multiple sclerosis

T2 - prominent expression of the Fc-receptor CD64

AU - Neuteboom, Rinze F.

AU - Verbraak, Evert

AU - Wierenga-Wolf, Annet F.

AU - Voerman, Jane S. A.

AU - van Meurs, Marjan

AU - Swagemakers, Sigrid M.

AU - de Spek, Peter J. van

AU - Steegers, Eric A. P.

AU - de Groot, Christianne J. M.

AU - Laman, Jon D.

AU - Hintzen, Rogier Q.

PY - 2011/4

Y1 - 2011/4

N2 - Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration.Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes.Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy.Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls.Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions.

AB - Background: During the third trimester of pregnancy multiple sclerosis (MS) disease activity is reduced. It is not fully understood which factors mediate this disease amelioration.Objective: To study alterations of the monocyte transcriptome during pregnancy in MS patients, using a genomewide approach to identify differentially regulated genes.Methods: Women with MS and healthy controls were longitudinally studied, including a visit before pregnancy.Results: RNA-microarray analysis was performed in six patients. We found a significant increase of CD64 (Fc gamma receptor 1a, FcgR1a) during the third trimester compared with baseline, confirmed by RT-PCR in a group of ten patients. Analysis with Ingenuity software was performed using all genes expression of which was altered at least 1.5-fold in at least five out of six patients. Major networks that were altered during MS pregnancy were: cell-to-cell signalling and interaction, immune response, and cell signalling. From the genes selected for Ingenuity analysis, seven additional candidate genes, selected for their biological interest, were tested using RT-PCR in ten patients with MS and nine controls. We found an increased expression of JAK2 and STAT1 directly postpartum in patients with MS and in controls.Conclusion: The increased CD64 expression during pregnancy is indicative of enhanced innate immune functions.

KW - autoimmunity

KW - CD64

KW - immune tolerance

KW - innate immunity

KW - multiple sclerosis

KW - pregnancy

KW - BLOOD MONONUCLEAR-CELLS

KW - T-BET

KW - IMMUNOGLOBULIN-G

KW - HORMONE ESTRIOL

KW - GAMMA

KW - DISEASE

KW - PSTAT1

KW - PREECLAMPSIA

KW - ACTIVATION

KW - INFECTION

U2 - 10.1177/1352458510389628

DO - 10.1177/1352458510389628

M3 - Article

SN - 1352-4585

VL - 17

SP - 389

EP - 396

JO - Multiple sclerosis journal

JF - Multiple sclerosis journal

IS - 4

ER -

The monocyte transcriptome during pregnancy in multiple sclerosis: prominent expression of the Fc-receptor CD64

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Keywords

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