The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli

Eiso AB, Gea Schuurman-Wolters, Jonathan Reizer, Milton H. Saier, Klaas Dijkstra, Ruud M. Scheek, George T. Robillard

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Abstract

The assignment of the side-chain Nh IR resonances and the determination of the three-dimensional solution structure of the C10S mutant of enzyme IIBcellobiose (IIBcel) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli are presented. The side-chain resonances were assigned nearly completely using a variety of mostly heteronuclear NMR experiments, including HCCH-TOCSY, HCCH-COSY, and COCCH-TOCSY experiments as well as CBCACOHA, CBCA(CO)NH, and HBHA(CBCA)(CO)NH experiments.

In order to obtain the three-dimensional structure, NOE data were collected from N-15-NOESY-HSQC, C-13-HSQC-NOESY, and 2D NOE experiments. The distance restraints derived from these NOE data were used in distance geometry calculations followed by molecular dynamics and simulated annealing protocols. In an iterative procedure, additional NOE assignments were derived from the calculated structures and new structures were calculated. The final set of structures, calculated with approximately 2000 unambiguous and ambiguous distance restraints, has an rms deviation of 1.1 Angstrom, on C alpha atoms. IIBcel consists of a four stranded parallel beta-sheet, in the order 2134. The sheet is flanked with two and three alpha-helices on either side. Residue 10, a cysteine in the wild-type enzyme, which is phosphorylated during the catalytic cycle, is located at the end of the first beta-strand. A loop that is proposed to be involved in the binding of the phosphoryl-group follows the cysteine. The loop appears to be disordered in the unphosphorylated state.

Original languageEnglish
Pages (from-to)304-314
Number of pages11
JournalProtein Science
Volume6
Issue number2
Publication statusPublished - Feb-1997

Keywords

  • ambiguous restraints
  • cellobiose
  • distance geometry
  • NMR
  • phosphocysteine
  • phosphoenolpyruvate-dependent phosphotransferase system
  • protein structure determination
  • PROTEIN-TYROSINE-PHOSPHATASE
  • PULSED-FIELD GRADIENTS
  • BACTERIAL PHOSPHOENOLPYRUVATE
  • GLUCOSE-TRANSPORTER
  • SECONDARY STRUCTURE
  • CRYSTAL-STRUCTURE
  • LARGER PROTEINS
  • SPECTROSCOPY
  • H2O
  • PHOSPHOCYSTEINE

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