The ntp operon encoding the Na+V-ATPase of the thermophile Caloramator fervidus

Trees Ubbink-Kok, Jeroen Nijland, Dirk-Jan Slotboom, Juke S. Lolkema*

*Corresponding author for this work

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Abstract

The V-type ATPase of the thermophile Caloramator fervidus is an ATP-driven Na+ pump. The nucleotide sequence of the ntpFIKECGABD operon containing the structural genes coding for the nine subunits of the enzyme complex was determined. The identity of the proteins in two pairs of subunits (D, E and F, G) that have very similar mobilities on SDS-PAGE of the purified complex (24.3 and 22.7 kDa, and 12.3 and 11.6 kDa) was established by tryptic digestion of the protein bands followed by mass spectrometric analysis of the peptides.

Original languageEnglish
Pages (from-to)513-517
Number of pages5
JournalArchives of Microbiology
Volume186
Issue number6
DOIs
Publication statusPublished - Dec-2006

Keywords

  • ntp Operon
  • V-ATPase
  • thermophile
  • Caloramator fervidus
  • molecular motor
  • central stalk
  • BACTERIUM CLOSTRIDIUM FERVIDUS
  • V-TYPE ATPASE
  • ELECTRON-MICROSCOPY
  • THERMUS-THERMOPHILUS
  • SUBUNIT ARRANGEMENT
  • ENTEROCOCCUS-HIRAE
  • PERIPHERAL STALK
  • STATOR STRUCTURE
  • COMPLEX
  • PURIFICATION

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