The origin of marginal zone B cells in the rat

PM Dammers*, NK de Boer, GJ Deenen, P Nieuwenhuis, FGM Kroese

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

45 Citations (Scopus)

Abstract

The marginal zone is a unique compartment that is only found in the spleen. Rat marginal zone B cells (MZ-B) can be distinguished from other B cells, e.g. recirculating follicular B cells (RF-B), by several phenotypic characteristics. Typically MZ-B cells are surface (s)IgM(hi), sIgD(lo) and CD45R(B220)(lo), whereas RF-B cells are sIgM(lo), sIgD(hi) and CD45R(hi). In addition, MZ-B cells stain strongly with HIS57, a newly developed monoclonal antibody. The developmental pathway and origin of MZ-B cells are not exactly known. However, previous studies indicate that recirculating (i.e. thoracic duct) B cells can give rise to MZ-B cells. Here the origin of (naive) WIZ-B cells was studied using adriamycin (doxorubicin)-induced B cell depletion. Using three-color flow cytometry and immunohistology we show that 2 days after a single i.v. injection of the anti-tumor drug adriamycin only RF-B cells can be detected, while all other B cell subpopulations are depleted, including all bone marrow precursor B cells. By studying the sequential reappearance of various B cell subsets and their precursors after adriamycin administration we show that MZ-B cells and the splenic marginal zone can be detected at a time point at which newly generated B cells (immature B cells) are not yet present. Given the observation that only RF-B cells were present at this time, we conclude that RF-B cells are the immediate MZ-B precursor cells.

Original languageEnglish
Pages (from-to)1522-1531
Number of pages10
JournalEuropean Journal of Immunology
Volume29
Issue number5
Publication statusPublished - May-1999

Keywords

  • B cell
  • marginal zone
  • differentiation
  • adriamycin
  • rat
  • PERIPHERAL LYMPHOID-TISSUES
  • GERMINAL CENTER FORMATION
  • ANTIBODY-RESPONSES
  • BONE-MARROW
  • SPLEEN
  • IGD
  • ACTIVATION
  • MEMORY
  • KINETICS
  • ANTIGENS

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