The patterned assembly and stepwise Vps4-mediated disassembly of composite ESCRT-III polymers drives archaeal cell division

Fredrik Hurtig, Thomas C Q Burgers, Alice Cezanne, Xiuyun Jiang*, Frank N Mol, Jovan Traparić, Andre Arashiro Pulschen, Tim Nierhaus, Gabriel Tarrason-Risa, Lena Harker-Kirschneck, Jan Löwe, Anđela Šarić*, Rifka Vlijm*, Buzz Baum

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

ESCRT-III family proteins form composite polymers that deform and cut membrane tubes in the context of a wide range of cell biological processes across the tree of life. In reconstituted systems, sequential changes in the composition of ESCRT-III polymers induced by the AAA-adenosine triphosphatase Vps4 have been shown to remodel membranes. However, it is not known how composite ESCRT-III polymers are organized and remodeled in space and time in a cellular context. Taking advantage of the relative simplicity of the ESCRT-III-dependent division system in Sulfolobus acidocaldarius, one of the closest experimentally tractable prokaryotic relatives of eukaryotes, we use super-resolution microscopy, electron microscopy, and computational modeling to show how CdvB/CdvB1/CdvB2 proteins form a precisely patterned composite ESCRT-III division ring, which undergoes stepwise Vps4-dependent disassembly and contracts to cut cells into two. These observations lead us to suggest sequential changes in a patterned composite polymer as a general mechanism of ESCRT-III-dependent membrane remodeling.

Original languageEnglish
Article numbereade5224
Number of pages12
JournalScience Advances
Volume9
Issue number11
DOIs
Publication statusPublished - 17-Mar-2023

Keywords

  • Endosomal Sorting Complexes Required for Transport/metabolism
  • Archaea/metabolism
  • Polymers
  • Cell Division

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