The Relation between Oral Candida Load and Bacterial Microbiome Profiles in Dutch Older Adults

Eefje A. Kraneveld*, Mark J. Buijs, Marc J. Bonder, Marjolein Visser, Bart J. F. Keijser, Wim Crielaard, Egija Zaura

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years was established relative to the bacterial load by quantitative PCR analysis of the Internal Transcribed (ITS) region (Candida) and 16S rDNA gene (bacteria). The salivary microbiome was assessed using barcoded pyrosequencing of the bacterial hypervariable regions V5-V7 of 16S rDNA. Sequencing data was preprocessed by denoising and chimera removal, clustered in Operational Taxonomic Units (OTUs) and assigned to taxonomy. Both OTU-based (PCA, diversity statistics) and phylogeny-based analyses (UniFrac, PCoA) were performed. Saliva of Dutch older adults contained 0-4 x 10(8) CFU/mL Candida with a median Candida load of 0.06%. With increased Candida load the diversity of the salivary microbiome decreased significantly (p

Original languageEnglish
Article numbere42770
Number of pages8
JournalPLoS ONE
Volume7
Issue number8
DOIs
Publication statusPublished - 10-Aug-2012
Externally publishedYes

Keywords

  • ALKALI PRODUCTION
  • ACID PRODUCTION
  • PCR ASSAYS
  • ALBICANS
  • DIVERSITY
  • EPIDEMIOLOGY
  • COMMUNITIES
  • SALIVARY
  • CARIES
  • STREPTOCOCCI

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