Abstract
Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophobic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane proteins was investigated. A high concentration of hydrophobized dextrans protected the liposomes against detergent degradation, decreased the fluidity of the membranes, prevented fusion of the liposomes and enhanced their stability. Proteoliposomes containing beef heart cytochrome-c oxidase and the lactose transport carrier of E. coli were similarly affected by coating with the dextrans. Under these conditions both membrane proteins were still active. Long-term stability of the coated liposomes was obtained only in the absence of the integral membrane proteins.
Original language | English |
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Pages (from-to) | 23-30 |
Number of pages | 8 |
Journal | Biochimica et biophysica acta |
Volume | 1106 |
Issue number | 1 |
DOIs | |
Publication status | Published - 29-Apr-1992 |
Keywords
- PROTEOLIPOSOME
- INTEGRAL MEMBRANE PROTEIN
- DEXTRAN DERIVATIVE
- HYDROPHOBIC ANCHOR GROUP
- PROTON-MOTIVE FORCE
- BIOLOGICAL-MEMBRANES
- ESCHERICHIA-COLI
- CYTOCHROME-C
- VESICLES
- PROTEIN
- KINETICS
- OXIDASE
- BINDING
- FUSION