The Staphylococcus aureus proteome

Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher

Research output: Contribution to journalReview articleAcademicpeer-review

22 Citations (Scopus)

Abstract

Staphylococcus aureus is a Gram-positive commensal bacterium that is regarded as a major threat for modern health care systems. This relates both to the ability of S. aureus to overcome antibiotic therapy by developing high-level resistance against multiple antibiotics and this bacterium's extensive arsenal of virulence factors. Understanding the mechanisms of resistance and functional studies on stress and starvation responses are the main goals of proteomics in staphylococcal research. This review high-lights recent advances in gel-based and gel-free proteomics analyses of S. aureus and pinpoints the importance of location-specific proteomics studies targeting the cytosol, the membrane, the cell surface and the extracellular milieu in combination with integrated global proteome studies. Emerging hot topics in staphylococcal proteomics are discussed with special focus on in vivo proteomics, membrane vesicles, biofilm formation and the acquisition of absolute proteome data for systems biological modeling approaches.

Original languageEnglish
Pages (from-to)110-120
Number of pages11
JournalInternational journal of medical microbiology
Volume304
Issue number2
DOIs
Publication statusPublished - Mar-2014

Keywords

  • Physiological proteomics
  • Entire proteome
  • 2D gel electrophoresis
  • MS-based proteomics
  • Stress/starvation responses
  • Virulence factors
  • Surfacome
  • Membrane vesicles
  • Biofilms
  • GRAM-POSITIVE BACTERIA
  • PHENOL-SOLUBLE MODULINS
  • HOST-PATHOGEN INTERACTIONS
  • ANAEROBIC GENE-EXPRESSION
  • COMMUNITY-ASSOCIATED MRSA
  • BACILLUS-SUBTILIS
  • 2-COMPONENT SYSTEM
  • CELL-SURFACE
  • GLUCOSE STARVATION
  • MEMBRANE-VESICLES

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