The structure of spinach Photosystem I studied by electron microscopy

Egbert J. Boekema; R. Max Wynn; Richard Malkin

doi:10.1016/0005-2728(90)90177-6

The structure of spinach Photosystem I studied by electron microscopy

Egbert J. Boekema, R. Max Wynn, Richard Malkin

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

Original language	English
Pages (from-to)	49-56
Number of pages	8
Journal	Biochimica et biophysica acta
Volume	1017
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(90)90177-6
Publication status	Published - 15-May-1990

Keywords

(Spinach)
Image analysis
Electron microscopy
Photosystem I structure

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title = "The structure of spinach Photosystem I studied by electron microscopy",

abstract = "The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.",

keywords = "(Spinach), Image analysis, Electron microscopy, Photosystem I structure",

author = "Boekema, {Egbert J.} and Wynn, {R. Max} and Richard Malkin",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen. Groningen Biomolecular Sciences and Biotechnology Institute",

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T1 - The structure of spinach Photosystem I studied by electron microscopy

AU - Boekema, Egbert J.

AU - Wynn, R. Max

AU - Malkin, Richard

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen. Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1990/5/15

Y1 - 1990/5/15

N2 - The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

AB - The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

KW - (Spinach)

KW - Image analysis

KW - Electron microscopy

KW - Photosystem I structure

U2 - 10.1016/0005-2728(90)90177-6

DO - 10.1016/0005-2728(90)90177-6

M3 - Article

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EP - 56

JO - Biochimica et biophysica acta

JF - Biochimica et biophysica acta

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ER -