The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges

Henno W. van den Hooven; Fija M. Lagerwerf; Wigger Heerma; Johan Haverkamp; Jean-Christophe Piard; Cornelis W. Hilbers; Roland J. Siezen; Oscar P. Kuipers; Harry S. Rollema

doi:10.1016/0014-5793(96)00771-5

The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges

Henno W. van den Hooven, Fija M. Lagerwerf, Wigger Heerma, Johan Haverkamp, Jean-Christophe Piard, Cornelis W. Hilbers, Roland J. Siezen, Oscar P. Kuipers, Harry S. Rollema

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic

47 Citations (Scopus)

204 Downloads (Pure)

Abstract

The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.

Original language	English
Number of pages	6
Journal	FEBS Letters
Volume	391
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(96)00771-5
Publication status	Published - 1996

Keywords

Mass spectrometry
NMR
Cyanogen bromide cleavage
Post-translational modification
Bacteriocin
Lanthionine-containing polypeptide

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10.1016/0014-5793(96)00771-5

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Cite this

@article{787d93cf270544b9bfc6ccd8363ecf0f,

title = "The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges",

abstract = "The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.",

keywords = "Mass spectrometry, NMR, Cyanogen bromide cleavage, Post-translational modification, Bacteriocin, Lanthionine-containing polypeptide",

author = "Hooven, {Henno W. van den} and Lagerwerf, {Fija M.} and Wigger Heerma and Johan Haverkamp and Jean-Christophe Piard and Hilbers, {Cornelis W.} and Siezen, {Roland J.} and Kuipers, {Oscar P.} and Rollema, {Harry S.}",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1996",

doi = "10.1016/0014-5793(96)00771-5",

language = "English",

volume = "391",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "3",

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The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis : location of the thioether bridges. / Hooven, Henno W. van den; Lagerwerf, Fija M.; Heerma, Wigger; Haverkamp, Johan; Piard, Jean-Christophe; Hilbers, Cornelis W.; Siezen, Roland J.; Kuipers, Oscar P.; Rollema, Harry S.

In: FEBS Letters, Vol. 391, No. 3, 1996.

Research output: Contribution to journal › Article › Academic

TY - JOUR

T1 - The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis

T2 - location of the thioether bridges

AU - Hooven, Henno W. van den

AU - Lagerwerf, Fija M.

AU - Heerma, Wigger

AU - Haverkamp, Johan

AU - Piard, Jean-Christophe

AU - Hilbers, Cornelis W.

AU - Siezen, Roland J.

AU - Kuipers, Oscar P.

AU - Rollema, Harry S.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1996

Y1 - 1996

N2 - The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.

AB - The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.

KW - Mass spectrometry

KW - NMR

KW - Cyanogen bromide cleavage

KW - Post-translational modification

KW - Bacteriocin

KW - Lanthionine-containing polypeptide

U2 - 10.1016/0014-5793(96)00771-5

DO - 10.1016/0014-5793(96)00771-5

M3 - Article

VL - 391

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -