The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: location of the thioether bridges

Henno W. van den Hooven, Fija M. Lagerwerf, Wigger Heerma, Johan Haverkamp, Jean-Christophe Piard, Cornelis W. Hilbers, Roland J. Siezen, Oscar P. Kuipers, Harry S. Rollema

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Abstract

The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis. This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.
Original languageEnglish
Number of pages6
JournalFEBS Letters
Volume391
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • Mass spectrometry
  • NMR
  • Cyanogen bromide cleavage
  • Post-translational modification
  • Bacteriocin
  • Lanthionine-containing polypeptide

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