The transfer of protein crystals from their original mother liquor to a solution with a completely different precipitant

Herman A. Schreuder*, Hillie Groendijk, Jan M. Van der Laan, Rik K. Wierenga

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    A procedure is described for the transfer of protein crystals from an ammonium sulfate‐containing mother liquor to a solution with another precipitant, such as polyethylene glycol. The suitable concentration of the alternative precipitant is established via a novel protocol, using a hanging‐drop equilibration method. This crystal transfer procedure is illustrated by experiments with crystals of trypanosomal triosephosphate isomerase and bacterial p ‐hydroxybenzoate hydroxylase, but it might have more general applicability.
    Original languageEnglish
    Pages (from-to)426-429
    Number of pages4
    JournalJournal of Applied Crystallography
    Volume21
    DOIs
    Publication statusPublished - 1-Oct-1988

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