The Use of Scanning Electron Microscopy (SEM) in Visualizing the Root Canal Biofilm

Linda Peters, Brandon Peterson, David E. Jaramillo, Lucas van der Sluis

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

26 Citations (Scopus)


Apical periodontitis is caused by microorganisms in planktonic or biofilm state present in the root canal system and/or attached to the outer apical root surface. Knowledge about the microorganisms and biofilm structure within and external to the root canal system is important in order to effectively treat apical periodontitis. Scanning electron microscopy (SEM) has been used to visualize and morphologically describe the presence of biofilm and microorganisms associated with teeth with apical periodontitis. This chapter provides a short outline of the applications of SEM in endodontics with the intention to describe the benefits and shortcomings of this microscopic technique.
Original languageEnglish
Title of host publicationThe Root Canal Biofilm
Number of pages15
ISBN (Electronic)978-3-662-47415-0
ISBN (Print)978-3-662-47414-3
Publication statusPublished - 2015

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