The value of thymus and activation related chemokine immunohistochemistry in classic Hodgkin lymphoma diagnostics

Melvin Kilsdonk, Carlijn Veldman, Stefano Rosati, Wouter Plattel, Arjan Diepstra*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
13 Downloads (Pure)

Abstract

Aims: Classic Hodgkin lymphoma (cHL) should be distinguished from its wide variety of histological mimics, including reactive conditions and mature B and T cell neoplasms. Thymus and activation-related chemokine (TARC) is produced in extremely high quantities by the Hodgkin/Reed–Sternberg (HRS) tumour cells and is largely responsible for the attraction of CD4+ T cells into the cHL tumour micro-environment. In the current study we evaluated the diagnostic potential of TARC immunohistochemistry in daily practice in a tertiary referral centre in the Netherlands. Methods and results: A total of 383 cases, approximately half of which were cHL mimics, were prospectively evaluated in the period from June 2014 to November 2020. In 190 cHL cases, 92% were TARC-positive and the majority of cases showed strong and highly specific staining in all HRS cells (77%). In most cases, TARC could discriminate between nodular lymphocyte-predominant and lymphocyte-rich Hodgkin lymphoma. HRS-like cells in mature lymphoid neoplasms were rarely positive (6.4%) and there was no TARC staining at all in 64 reactive lymphadenopathies. Conclusions: TARC immunohistochemistry has great value in differentiating between cHL and its mimics, including nodular lymphocyte-predominant Hodgkin lymphoma, reactive lymphadenopathies and mature lymphoid neoplasms with HRS-like cells.

Original languageEnglish
Pages (from-to)495-503
Number of pages9
JournalHistopathology
Volume82
Issue number3
Early online date23-Nov-2022
DOIs
Publication statusPublished - Feb-2023

Keywords

  • CCL17
  • differential diagnostics
  • Hodgkin lymphoma
  • immunohistochemistry
  • TARC

Cite this