The yeast Saccharomyces cerevisiae: An overview of methods to study autophagy progression

Elizabeth Delorme-Axford, Rodrigo Soares Guimaraes, Fulvio Reggiori, Daniel J. Klionsky*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

42 Citations (Scopus)

Abstract

Macroautophagy (hereafter autophagy) is a highly evolutionarily conserved process essential for sustaining cellular integrity, homeostasis, and survival. Most eukaryotic cells constitutively undergo autophagy at a low basal level. However, various stimuli, including starvation, organelle deterioration, stress, and pathogen infection, potently upregulate autophagy. The hallmark morphological feature of autophagy is the formation of the double-membrane vesicle known as the autophagosome. In yeast, flux through the pathway culminates in autophagosome-vacuole fusion, and the subsequent degradation of the resulting autophagic bodies and cargo by vacuolar hydrolases, followed by efflux of the breakdown products. Importantly, aberrant autophagy is associated with diverse human pathologies. Thus, there is a need for ongoing work in this area to further understand the cellular factors regulating this process. The field of autophagy research has grown exponentially in recent years, and although numerous model organisms are being used to investigate autophagy, the baker's yeast Saccharomyces cerevisiae remains highly relevant, as there are significant and unique benefits to working with this organism. In this review, we will focus on the current methods available to evaluate and monitor autophagy in S. cerevisiae, which in several cases have also been subsequently exploited in higher eukaryotes.

Original languageEnglish
Pages (from-to)3-12
Number of pages10
JournalMethods
Volume75
DOIs
Publication statusPublished - 15-Mar-2015

Keywords

  • Atg8
  • Autophagosome
  • Mitophagy
  • PAS
  • Phagophore
  • Vacuole
  • VACUOLE TARGETING PATHWAY
  • PHOSPHATIDYLINOSITOL 3-PHOSPHATE ASYMMETRIES
  • PHAGOPHORE ASSEMBLY SITE
  • ISOLATED RAT HEPATOCYTES
  • PROTEIN-KINASE COMPLEX
  • SIGNALING PATHWAYS
  • ENDOPLASMIC-RETICULUM
  • MONITORING AUTOPHAGY
  • ELECTRON-MICROSCOPY
  • SELECTIVE AUTOPHAGY

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