Thermotolerance and nuclear protein aggregation: Protection against initial damage or better recovery?

G. J. J. Stege*, J. F. Brunsting, H. H. Kampinga, A. W. T. Konings

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    34 Citations (Scopus)

    Abstract

    Heat-induced nuclear protein aggregation and subsequent disaggregation were measured in nonpreheated and preheated (thermotolerant) HeLa S3 cells. The effect of thermotolerance on the formation of and recovery from heat-induced nuclear protein aggregates was related to the cellular levels of hsp27, hsp60, hsp70, hsp70, and hsp90. Cells heated at different time points after the thermotolerance trigger showed various levels of protection against heat-induced nuclear protein aggregation. This protection, however, did not parallel the development and decay of thermotolerance on cell survival. The protection was maximal when the thermotolerance level already had started to decay. The level of protection against nuclear protein aggregation did however parallel the cellular level of hsp70 indicating that hsp70 may be involved in this process. At all stages during the development and decay, thermotolerant cells showed a more rapid recovery (disaggregation) from the heat-induced nuclear protein aggregates than non-thermotolerant cells. The rates of disaggregation during development and decay of thermotolerance paralleled the cellular levels of hsp27 suggesting that hsp27 is somehow involved in this recovery process from heat-induced nuclear protein aggregates. The total cellular levels of none of the individual hsp's completely correlate with development and decay of thermotolerance, indicating that overexpression of any of these hsp's alone does not determine the level of thermotolerance. Clonogenic cell survival paralleled the rates of disaggregation, leading to the notion that recovery processes are the most important determinant for the thermotolerant state of Hela S3 cells. The best correlation with clonogenic survival was found when both initial aggregation and subsequent disaggregation were taken into account, suggesting that the combined action of various hsp's in these two processes have to be included in thermotolerance development and decay. (C) 1995 Wiley-Liss, Inc.

    Original languageEnglish
    Pages (from-to)579-586
    Number of pages8
    JournalJournal of Cellular Physiology
    Volume164
    Issue number3
    DOIs
    Publication statusPublished - Sep-1995

    Keywords

    • HEAT-SHOCK PROTEINS
    • HELA S3 CELLS
    • MAMMALIAN-CELLS
    • STRESS PROTEINS
    • ABNORMAL PROTEINS
    • UNCOATING PROTEIN
    • GENE-EXPRESSION
    • ATP HYDROLYSIS
    • HSP27 GENE
    • HYPERTHERMIA

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