Three-dimensional reconstruction of the guinea pig inner ear, comparison of OPFOS and light microscopy, applications of 3D reconstruction

R. Hofman*, J. M. Segenhout, H. P. Wit

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    Three-dimensional (3D) reconstruction of anatomical structures can give additional insight into the morphology and function of these structures. We compare 3D reconstructions of the guinea pig inner ear, using light microscopy and orthogonal plane fluorescence optical sectioning microscopy. Applications of 3D reconstruction of the inner ear are further explored. For each method two bullas were prepared for 3D reconstruction. Both methods are explained. In general, the 3D reconstructions using orthogonal plane fluorescence optical sectioning microscopy are superior to light microscopy. The exact spiral shape of the cochlea could be reconstructed using orthogonal plane fluorescence optical sectioning microscopy and the length of the basilar membrane measured. When a resolution of 20 mu m is sufficient, orthogonal plane fluorescence optical sectioning microscopy is a superior technique for 3D reconstruction of inner ear structures in animals.

    Original languageEnglish
    Pages (from-to)251-257
    Number of pages7
    JournalJournal of Microscopy
    Volume233
    Issue number2
    DOIs
    Publication statusPublished - Feb-2009

    Keywords

    • Cochlea
    • endolymphatic system
    • imaging
    • three dimensional
    • ROUND WINDOW MEMBRANE
    • COCHLEAR AQUEDUCT
    • IMAGES

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