Tissue reactions to collagen scaffolds in the oral mucosa and skin of rats: Environmental and mechanical factors

Richard G. Jansen; Toin H. van Kuppevelt; Willeke F. Daamen; Anne Marie KuiJpers-Jagtman; Johannes W. Von den Hoff

doi:10.1016/j.archoralbio.2007.11.003

Tissue reactions to collagen scaffolds in the oral mucosa and skin of rats: Environmental and mechanical factors

Richard G. Jansen, Toin H. van Kuppevelt, Willeke F. Daamen, Anne Marie KuiJpers-Jagtman, Johannes W. Von den Hoff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

Objective: To compare the tissue reactions to implanted Collagen scaffolds in the palate and the skin of rats.

Design: Crosslinked collagen scaffolds were implanted submucoperiosteally in the palate, and subcutaneously on the skull and on the back of 25 rats and evaluated after up to 16 weeks. On H&E-stained sections, the cell density and the number of giant cells within the scaffolds were determined. Blood vessels, inflammatory cells, and myofibroblasts were detected by immunohistochemistry.

Results: A faster ingrowth of myofibroblasts and blood vessels in the palate was found during the first week compared with the skin. A more severe inflammatory response was initially found in the back skin. Furthermore, about twice as much giant cells were present in these scaffolds.

Conclusion: The oral environment seems to promote the ingrowth of myofibroblasts and blood vessels into the scaffolds. Mechanical stimuli seem to enhance the initial inflammatory response. Overall, the scaffolds were gradually integrated within the host tissue, eliciting only a transient inflammatory response. The scaffolds were biocompatible and are promising for future applications in oral surgery.

Original language	English
Pages (from-to)	376-387
Number of pages	12
Journal	Archives of Oral Biology
Volume	53
Issue number	4
DOIs	https://doi.org/10.1016/j.archoralbio.2007.11.003
Publication status	Published - Apr-2008
Externally published	Yes

Keywords

collagen
scaffold
wound healing
animal model
in vivo test
HEALING PALATAL WOUNDS
ENDOTHELIAL GROWTH-FACTOR
PERCUTANEOUS DEVICES
SCAR FORMATION
BEAGLE DOG
MYOFIBROBLASTS
FIBROBLASTS
CONTRACTION
MATRICES
ACTIN

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Tissue reactions to collagen scaffolds in the oral mucosa and skin of rats
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title = "Tissue reactions to collagen scaffolds in the oral mucosa and skin of rats: Environmental and mechanical factors",

abstract = "Objective: To compare the tissue reactions to implanted Collagen scaffolds in the palate and the skin of rats.Design: Crosslinked collagen scaffolds were implanted submucoperiosteally in the palate, and subcutaneously on the skull and on the back of 25 rats and evaluated after up to 16 weeks. On H&E-stained sections, the cell density and the number of giant cells within the scaffolds were determined. Blood vessels, inflammatory cells, and myofibroblasts were detected by immunohistochemistry.Results: A faster ingrowth of myofibroblasts and blood vessels in the palate was found during the first week compared with the skin. A more severe inflammatory response was initially found in the back skin. Furthermore, about twice as much giant cells were present in these scaffolds.Conclusion: The oral environment seems to promote the ingrowth of myofibroblasts and blood vessels into the scaffolds. Mechanical stimuli seem to enhance the initial inflammatory response. Overall, the scaffolds were gradually integrated within the host tissue, eliciting only a transient inflammatory response. The scaffolds were biocompatible and are promising for future applications in oral surgery.",

keywords = "collagen, scaffold, wound healing, animal model, in vivo test, HEALING PALATAL WOUNDS, ENDOTHELIAL GROWTH-FACTOR, PERCUTANEOUS DEVICES, SCAR FORMATION, BEAGLE DOG, MYOFIBROBLASTS, FIBROBLASTS, CONTRACTION, MATRICES, ACTIN",

author = "Jansen, {Richard G.} and {van Kuppevelt}, {Toin H.} and Daamen, {Willeke F.} and KuiJpers-Jagtman, {Anne Marie} and {Von den Hoff}, {Johannes W.}",

year = "2008",

month = apr,

doi = "10.1016/j.archoralbio.2007.11.003",

language = "English",

volume = "53",

pages = "376--387",

journal = "Archives of Oral Biology",

issn = "0003-9969",

publisher = "PERGAMON-ELSEVIER SCIENCE LTD",

number = "4",

}

TY - JOUR

T1 - Tissue reactions to collagen scaffolds in the oral mucosa and skin of rats

T2 - Environmental and mechanical factors

AU - Jansen, Richard G.

AU - van Kuppevelt, Toin H.

AU - Daamen, Willeke F.

AU - KuiJpers-Jagtman, Anne Marie

AU - Von den Hoff, Johannes W.

PY - 2008/4

Y1 - 2008/4

N2 - Objective: To compare the tissue reactions to implanted Collagen scaffolds in the palate and the skin of rats.Design: Crosslinked collagen scaffolds were implanted submucoperiosteally in the palate, and subcutaneously on the skull and on the back of 25 rats and evaluated after up to 16 weeks. On H&E-stained sections, the cell density and the number of giant cells within the scaffolds were determined. Blood vessels, inflammatory cells, and myofibroblasts were detected by immunohistochemistry.Results: A faster ingrowth of myofibroblasts and blood vessels in the palate was found during the first week compared with the skin. A more severe inflammatory response was initially found in the back skin. Furthermore, about twice as much giant cells were present in these scaffolds.Conclusion: The oral environment seems to promote the ingrowth of myofibroblasts and blood vessels into the scaffolds. Mechanical stimuli seem to enhance the initial inflammatory response. Overall, the scaffolds were gradually integrated within the host tissue, eliciting only a transient inflammatory response. The scaffolds were biocompatible and are promising for future applications in oral surgery.

AB - Objective: To compare the tissue reactions to implanted Collagen scaffolds in the palate and the skin of rats.Design: Crosslinked collagen scaffolds were implanted submucoperiosteally in the palate, and subcutaneously on the skull and on the back of 25 rats and evaluated after up to 16 weeks. On H&E-stained sections, the cell density and the number of giant cells within the scaffolds were determined. Blood vessels, inflammatory cells, and myofibroblasts were detected by immunohistochemistry.Results: A faster ingrowth of myofibroblasts and blood vessels in the palate was found during the first week compared with the skin. A more severe inflammatory response was initially found in the back skin. Furthermore, about twice as much giant cells were present in these scaffolds.Conclusion: The oral environment seems to promote the ingrowth of myofibroblasts and blood vessels into the scaffolds. Mechanical stimuli seem to enhance the initial inflammatory response. Overall, the scaffolds were gradually integrated within the host tissue, eliciting only a transient inflammatory response. The scaffolds were biocompatible and are promising for future applications in oral surgery.

KW - collagen

KW - scaffold

KW - wound healing

KW - animal model

KW - in vivo test

KW - HEALING PALATAL WOUNDS

KW - ENDOTHELIAL GROWTH-FACTOR

KW - PERCUTANEOUS DEVICES

KW - SCAR FORMATION

KW - BEAGLE DOG

KW - MYOFIBROBLASTS

KW - FIBROBLASTS

KW - CONTRACTION

KW - MATRICES

KW - ACTIN

U2 - 10.1016/j.archoralbio.2007.11.003

DO - 10.1016/j.archoralbio.2007.11.003

M3 - Article

SN - 0003-9969

VL - 53

SP - 376

EP - 387

JO - Archives of Oral Biology

JF - Archives of Oral Biology

IS - 4

ER -

Tissue reactions to collagen scaffolds in the oral mucosa and skin of rats: Environmental and mechanical factors

Abstract

Keywords

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