Tools for genetic engineering of the yeast Hansenula polymorpha

Ruchi Saraya, Loknath Gidijala, Marten Veenhuis, Ida J van der Klei*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

11 Citations (Scopus)


Hansenula polymorpha is a methylotrophic yeast species that has favorable properties for heterologous protein production and metabolic engineering. It provides an attractive expression platform with the capability to secrete high levels of commercially important proteins. Over the past few years many efforts have led to advances in the development of this microbial host including the generation of expression vectors containing strong constitutive or inducible promoters and a large array of dominant and auxotrophic markers. Moreover, highly efficient transformation procedures used to generate genetically stable strains are now available. Here, we describe these tools as well as the methods for genetic engineering of H. polymorpha.

Original languageEnglish
Title of host publicationYeast Metabolic Engineering
Subtitle of host publicationMethods and Protocols
EditorsValeria Mapelli
PublisherSpringer Verlag
Number of pages20
ISBN (Print)978-1-4939-0563-8
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Genetic engineering
  • Hansenula polymorpha
  • Methylotrophic yeast
  • Metabolic engineering
  • Heterologous protein production

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