Topographical distribution of a membrane-inserted fluorescent phospholipid analogue during cell fusion

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    Abstract

    Potential alterations in the transbilayer distribution of lipid molecules during cell-cell fusion were studied, using the fluorescent phospholipid analogue 1-acyl, 2-(N-4-nitrobenzo-2-oxa-1,3-diazole)-aminocaproyl phosphatidylcholine (C6-NBD-PC). The fluophore was inserted into the outer leaflet of the plasma membrane of Chinese hamster fibroblasts from an exogenous source and cell-cell fusion was induced either with Sendai virus or polyethylene glycol (PEG). After fusion, the cells were examined under a fluorescence microscope and the pool of tagged lipid molecules in the external monolayer was determined quantitatively. The results showed that in contrast to PEG-induced cell fusion, substantial redistribution of the lipid marker occurred when cell fusion was induced by Sendai virus and it was estimated that approx. 40% of exogenously supplied lipid was internalized. The possible mechanism causing lipid redistribution in the case of Sendai virus-induced cell fusion is discussed.

    Original languageEnglish
    Pages (from-to)482-488
    Number of pages7
    JournalExperimental Cell Research
    Volume144
    Issue number2
    Publication statusPublished - 1-Apr-1983

    Keywords

    • Animals
    • Cell Fusion
    • Cell Line
    • Cricetinae
    • Fibroblasts
    • Fluorescent Dyes
    • Liposomes
    • Membrane Lipids
    • Parainfluenza Virus 1, Human
    • Phosphatidylcholines
    • Polyethylene Glycols

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