Topologically fixed SecG is fully functional

Eli O. van der Sluis; E van der Vries; G Berrelkamp; N Nouwen; AJM Driessen

doi:10.1128/JB.188.3.1188-1190.2006

Topologically fixed SecG is fully functional

Eli O. van der Sluis, E van der Vries, G Berrelkamp, N Nouwen, AJM Driessen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

18 Citations (Scopus)

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Abstract

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

Original language	English
Pages (from-to)	1188-1190
Number of pages	3
Journal	Journal of Bacteriology
Volume	188
Issue number	3
DOIs	https://doi.org/10.1128/JB.188.3.1188-1190.2006
Publication status	Published - Feb-2006

Keywords

PROTEIN TRANSLOCATION MACHINERY
ESCHERICHIA-COLI
CROSS-LINKING
CYTOPLASMIC MEMBRANE
PREPROTEIN
COMPLEX
INVERSION
COMPONENT
SUBUNITS
ROLES

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title = "Topologically fixed SecG is fully functional",

abstract = "It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.",

keywords = "PROTEIN TRANSLOCATION MACHINERY, ESCHERICHIA-COLI, CROSS-LINKING, CYTOPLASMIC MEMBRANE, PREPROTEIN, COMPLEX, INVERSION, COMPONENT, SUBUNITS, ROLES",

author = "Sluis, {Eli O. van der} and {van der Vries}, E and G Berrelkamp and N Nouwen and AJM Driessen",

year = "2006",

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doi = "10.1128/JB.188.3.1188-1190.2006",

language = "English",

volume = "188",

pages = "1188--1190",

journal = "Journal of Bacteriology",

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T1 - Topologically fixed SecG is fully functional

AU - Sluis, Eli O. van der

AU - van der Vries, E

AU - Berrelkamp, G

AU - Nouwen, N

AU - Driessen, AJM

PY - 2006/2

Y1 - 2006/2

N2 - It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

AB - It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

KW - PROTEIN TRANSLOCATION MACHINERY

KW - ESCHERICHIA-COLI

KW - CROSS-LINKING

KW - CYTOPLASMIC MEMBRANE

KW - PREPROTEIN

KW - COMPLEX

KW - INVERSION

KW - COMPONENT

KW - SUBUNITS

KW - ROLES

U2 - 10.1128/JB.188.3.1188-1190.2006

DO - 10.1128/JB.188.3.1188-1190.2006

M3 - Article

SN - 0021-9193

VL - 188

SP - 1188

EP - 1190

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 3

ER -