Topologically fixed SecG is fully functional

Eli O. van der Sluis, E van der Vries, G Berrelkamp, N Nouwen, AJM Driessen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)
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Abstract

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

Original languageEnglish
Pages (from-to)1188-1190
Number of pages3
JournalJournal of Bacteriology
Volume188
Issue number3
DOIs
Publication statusPublished - Feb-2006

Keywords

  • PROTEIN TRANSLOCATION MACHINERY
  • ESCHERICHIA-COLI
  • CROSS-LINKING
  • CYTOPLASMIC MEMBRANE
  • PREPROTEIN
  • COMPLEX
  • INVERSION
  • COMPONENT
  • SUBUNITS
  • ROLES

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