It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.
|Number of pages||3|
|Journal||Journal of Bacteriology|
|Publication status||Published - Feb-2006|
- PROTEIN TRANSLOCATION MACHINERY
- CYTOPLASMIC MEMBRANE