Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis

Christian M. Franke; Kees J. Leenhouts; Alfred J. Haandrikman; Jan Kok; Gerard Venema; Koen Venema

Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis

Christian M. Franke, Kees J. Leenhouts, Alfred J. Haandrikman, Jan Kok, Gerard Venema, Koen Venema

Groningen Biomolecular Sciences and Biotechnology Institute

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Abstract

Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.

Original language	English
Pages (from-to)	1766-1769
Number of pages	4
Journal	Journal of Bacteriology
Volume	178
Issue number	6
Publication status	Published - Mar-1996

Keywords

ESCHERICHIA-COLI
MEMBRANE-PROTEINS
SUBSP LACTIS
CLONING
EXPRESSION
SEQUENCES
PLASMID
GENES

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@article{dc2290feb72043b184cd1e2b0659f493,

title = "Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis",

abstract = "Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.",

keywords = "ESCHERICHIA-COLI, MEMBRANE-PROTEINS, SUBSP LACTIS, CLONING, EXPRESSION, SEQUENCES, PLASMID, GENES",

author = "Franke, {Christian M.} and Leenhouts, {Kees J.} and Haandrikman, {Alfred J.} and Jan Kok and Gerard Venema and Koen Venema",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1996",

month = mar,

language = "English",

volume = "178",

pages = "1766--1769",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "AMER SOC MICROBIOLOGY",

number = "6",

}

TY - JOUR

T1 - Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis

AU - Franke, Christian M.

AU - Leenhouts, Kees J.

AU - Haandrikman, Alfred J.

AU - Kok, Jan

AU - Venema, Gerard

AU - Venema, Koen

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1996/3

Y1 - 1996/3

N2 - Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.

AB - Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.

KW - ESCHERICHIA-COLI

KW - MEMBRANE-PROTEINS

KW - SUBSP LACTIS

KW - CLONING

KW - EXPRESSION

KW - SEQUENCES

KW - PLASMID

KW - GENES

M3 - Article

VL - 178

SP - 1766

EP - 1769

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -