Abstract
Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.
Original language | English |
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Pages (from-to) | 1766-1769 |
Number of pages | 4 |
Journal | Journal of Bacteriology |
Volume | 178 |
Issue number | 6 |
Publication status | Published - Mar-1996 |
Keywords
- ESCHERICHIA-COLI
- MEMBRANE-PROTEINS
- SUBSP LACTIS
- CLONING
- EXPRESSION
- SEQUENCES
- PLASMID
- GENES