Towards design principles for determining the mechanical stability of proteins

Toni Hoffmann, Kasia Tych, Megan L. Hughes, David J. Brockwell, Lorna Dougan*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)

Abstract

The successful integration of proteins into bionanomaterials with specific and desired functions requires an accurate understanding of their material properties. Two such important properties are their mechanical stability and malleability. While single molecule manipulation techniques now routinely provide access to these, there is a need to move towards predictive tools that can rationally identify proteins with desired material properties. We provide a comprehensive review of the available experimental data on the single molecule characterisation of proteins using the atomic force microscope. We uncover a number of empirical relationships between the measured mechanical stability of a protein and its malleability, which provide a set of simple tools that might be employed to estimate properties of previously uncharacterised proteins.
Original languageEnglish
Pages (from-to)15767-15780
Number of pages14
JournalPhysical Chemistry Chemical Physics
Volume38
DOIs
Publication statusPublished - 2013
Externally publishedYes

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