Towards standardization of the human cytomegalovirus antigenemia assay

EAM Verschuuren; MC Harmsen; PC Limburg; W van der Bij; AP van den Berg; AMKDB Meedendorp; WJ van Son; T Hauw; Biomed 2 Study Grp

Towards standardization of the human cytomegalovirus antigenemia assay

EAM Verschuuren^*
, MC Harmsen
, PC Limburg
, W van der Bij
, AP van den Berg
, AMKDB Meedendorp
, WJ van Son
, T Hauw
, Biomed 2 Study Grp

^*Corresponding author for this work

Groningen Institute for Organ Transplantation (GIOT)

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

The Human Cytomegalovirus antigenemia (HCMV-Agemia) test has been accepted worldwide as a clinical tool in the diagnosis and management of HCMV-associated syndromes in immunocompromised patients. The many modifications proposed since the first description by our laboratory make standardisation of the HCMV-Agemia assay necessary to enable multicenter clinical trials. We report the initial work for standardization of the HCMV-Agemia ia assay. A sta nda rd protocol is proposed, the optimal distribution conditions are investigated and the results of the shipment of positive and negative test slides as well as of two sets of coded internal standard slides are discussed. The main conclusions are that standard slides can be distributed at room temperature and that the results of participating laboratories with the coded internal standard slides were strikingly similar in spite of differences in HCMV-Agemia protocols used by participating laboratories. Copyright (C) 2000 S. Karger AG, Basel.

Original language	English
Pages (from-to)	382-389
Number of pages	8
Journal	Intervirology
Volume	42
Issue number	5-6
Publication status	Published - 1999
Event	2nd Symposium on CMV-Related Immunopathology - , Netherlands Duration: 17-Sept-1999 → 18-Sept-1999

Keywords

cytomegalovirus
antigenemia
standardization
PERIPHERAL-BLOOD LEUKOCYTES
IMMEDIATE EARLY ANTIGEN
VIRUS
TRANSPLANTATION
INFECTION
VIREMIA
PP65

Handle.net

Persistent link

Cite this

@article{27858f3708df40e5b1a2d7ac7b0acbee,

title = "Towards standardization of the human cytomegalovirus antigenemia assay",

abstract = "The Human Cytomegalovirus antigenemia (HCMV-Agemia) test has been accepted worldwide as a clinical tool in the diagnosis and management of HCMV-associated syndromes in immunocompromised patients. The many modifications proposed since the first description by our laboratory make standardisation of the HCMV-Agemia assay necessary to enable multicenter clinical trials. We report the initial work for standardization of the HCMV-Agemia ia assay. A sta nda rd protocol is proposed, the optimal distribution conditions are investigated and the results of the shipment of positive and negative test slides as well as of two sets of coded internal standard slides are discussed. The main conclusions are that standard slides can be distributed at room temperature and that the results of participating laboratories with the coded internal standard slides were strikingly similar in spite of differences in HCMV-Agemia protocols used by participating laboratories. Copyright (C) 2000 S. Karger AG, Basel.",

keywords = "cytomegalovirus, antigenemia, standardization, PERIPHERAL-BLOOD LEUKOCYTES, IMMEDIATE EARLY ANTIGEN, VIRUS, TRANSPLANTATION, INFECTION, VIREMIA, PP65",

author = "EAM Verschuuren and MC Harmsen and PC Limburg and \{van der Bij\}, W and \{van den Berg\}, AP and AMKDB Meedendorp and \{van Son\}, WJ and T Hauw and \{Biomed 2 Study Grp\}",

year = "1999",

language = "English",

volume = "42",

pages = "382--389",

journal = "Intervirology",

issn = "0300-5526",

number = "5-6",

note = "2nd Symposium on CMV-Related Immunopathology ; Conference date: 17-09-1999 Through 18-09-1999",

}

TY - JOUR

T1 - Towards standardization of the human cytomegalovirus antigenemia assay

AU - Verschuuren, EAM

AU - Harmsen, MC

AU - Limburg, PC

AU - van der Bij, W

AU - van den Berg, AP

AU - Meedendorp, AMKDB

AU - van Son, WJ

AU - Hauw, T

AU - Biomed 2 Study Grp

PY - 1999

Y1 - 1999

N2 - The Human Cytomegalovirus antigenemia (HCMV-Agemia) test has been accepted worldwide as a clinical tool in the diagnosis and management of HCMV-associated syndromes in immunocompromised patients. The many modifications proposed since the first description by our laboratory make standardisation of the HCMV-Agemia assay necessary to enable multicenter clinical trials. We report the initial work for standardization of the HCMV-Agemia ia assay. A sta nda rd protocol is proposed, the optimal distribution conditions are investigated and the results of the shipment of positive and negative test slides as well as of two sets of coded internal standard slides are discussed. The main conclusions are that standard slides can be distributed at room temperature and that the results of participating laboratories with the coded internal standard slides were strikingly similar in spite of differences in HCMV-Agemia protocols used by participating laboratories. Copyright (C) 2000 S. Karger AG, Basel.

AB - The Human Cytomegalovirus antigenemia (HCMV-Agemia) test has been accepted worldwide as a clinical tool in the diagnosis and management of HCMV-associated syndromes in immunocompromised patients. The many modifications proposed since the first description by our laboratory make standardisation of the HCMV-Agemia assay necessary to enable multicenter clinical trials. We report the initial work for standardization of the HCMV-Agemia ia assay. A sta nda rd protocol is proposed, the optimal distribution conditions are investigated and the results of the shipment of positive and negative test slides as well as of two sets of coded internal standard slides are discussed. The main conclusions are that standard slides can be distributed at room temperature and that the results of participating laboratories with the coded internal standard slides were strikingly similar in spite of differences in HCMV-Agemia protocols used by participating laboratories. Copyright (C) 2000 S. Karger AG, Basel.

KW - cytomegalovirus

KW - antigenemia

KW - standardization

KW - PERIPHERAL-BLOOD LEUKOCYTES

KW - IMMEDIATE EARLY ANTIGEN

KW - VIRUS

KW - TRANSPLANTATION

KW - INFECTION

KW - VIREMIA

KW - PP65

M3 - Article

SN - 0300-5526

VL - 42

SP - 382

EP - 389

JO - Intervirology

JF - Intervirology

IS - 5-6

T2 - 2nd Symposium on CMV-Related Immunopathology

Y2 - 17 September 1999 through 18 September 1999

ER -

Towards standardization of the human cytomegalovirus antigenemia assay

Abstract

Keywords

Handle.net

Fingerprint

Cite this