TOXICOLOGICAL ANALYSIS OF WHOLE-BLOOD SAMPLES BY MEANS OF BOND-ELUT CERTIFY COLUMNS AND GAS-CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION

PGM ZWEIPFENNING*, AHCM WILDERINK, P HORSTHUIS, JP FRANKE, RA DEZEEUW

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

The application of Bond-Elut Certify solid-phase extraction columns to the systematic toxicological analysis of whole blood was evaluated. The reproducibility of the extraction was tested with thirteen drugs varying in physico-chemical properties. Analysis was performed with capillary gas chromatography with nitrogen-selective detection. The recoveries were reproducible, as long as other limiting factors, e.g., chromatographic behaviour or volatility, do not play a significant role. The effect of limiting chromatographic behaviour was studied in more detail with the more sensitive mass spectrometry with selected-ion monitoring after converting the extracted morphine into its ditrimethylsilyl derivative.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalJournal of Chromatography A
Volume674
Issue number1-2
Publication statusPublished - 15-Jul-1994

Keywords

  • SOLID-PHASE EXTRACTION
  • PLASMA
  • DRUGS
  • URINE

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