Tracking Down the Cause of Necrotizing Fasciitis in a Patient with Negative Cultures

Bastiaan Vierhout, Evert van Zanten, Guido Wisselink , Anna M. D. Kooistra-Smid, Alewijn Ott

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    Abstract

    In a patient suspected of lower abdominal necrotizing fasciitis cultures remained negative, possibly because broad spectrum antibiotics had already been given before tissue for culture was obtained. 16S-23S rDNANext Generation Sequencing showed that 99.7% of bacterial DNA was of Streptococcus pyogenes. 16S-23S rDNA can replace culture for identification of bacteria, also in polymicrobial infection.
    Original languageEnglish
    Article number2011
    Number of pages2
    JournalClinics in Surgery
    Volume3
    Publication statusPublished - 12-Jul-2018

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