trans-3-chloroacrylic acid dehalogenase from Pseudomonas pavonaceae 170 shares structural and mechanistic similarities with 4-oxalocrotonate tautomerase

GJ Poelarends, R Saunier, DB Janssen*

*Corresponding author for this work

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Abstract

The genes (caaD1 and caaD2) encoding the trans-3-chloroacrylic acid dehalogenase (CaaD) of the 1,3-dichloropropene-utilizing bacterium Pseudomonas pavonaceae 170 were cloned and heterologously expressed in Escherichia coli and Pseudomonas sp. strain GJ1, CaaD is a protein of 50 kDa that is composed of alpha -subunits of 75 amino acid residues and beta -subunits of 70 residues. It catalyzes the hydrolytic cleavage of the beta -vinylic carbon-chlorine bond in trans-3-chloroacrylic acid with a turnover number of 6.4 s(-1). On the basis of sequence similarity, oligomeric structure, and subunit size, CaaD appears to be related to 4-oxalocrotonate tautomerase (4-OT), This tautomerase consists of six identical subunits of 62 amino acid residues and catalyzes the isomerization of 2-oxo-4-hexene-1,6-dioate, via hydroxymuconate, to yield 2-oxo-3-hexene-1,6-dioate. In view of the oligomeric architecture of 4-OT, a trimer of homodimers, CaaD is postulated to be a hexameric protein that functions as a trimer of alpha beta -dimers. The sequence conservation between CaaD and 4-OT and site-directed mutagenesis experiments suggested that Pro-1 of the beta -subunit and Arg-11 of the alpha -subunit are active-site residues in CaaD, Pro-1 could act as the proton acceptor/donor, and Arg-ll is probably involved in carboxylate binding. Based on these findings, a novel dehalogenation mechanism is proposed for the CaaD-catalyzed reaction which does not involve the formation of a covalent enzyme-substrate intermediate.

Original languageEnglish
Pages (from-to)4269-4277
Number of pages9
JournalJournal of Bacteriology
Volume183
Issue number14
DOIs
Publication statusPublished - Jul-2001

Keywords

  • MIGRATION INHIBITORY FACTOR
  • AMINO-TERMINAL PROLINE
  • XANTHOBACTER-AUTOTROPHICUS GJ10
  • ACTIVE-SITE RESIDUES
  • HALIDE-BINDING-SITE
  • HALOALKANE DEHALOGENASE
  • L-2-HALOACID DEHALOGENASE
  • CRYSTAL-STRUCTURE
  • HALOACETATE HALIDOHYDROLASE
  • CATALYTIC MECHANISM

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