Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi

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Abstract

Filamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent secondary metabolite biosynthetic gene clusters (BGCs). Various strategies have been carried out to explore and mine this untapped source of bioactive molecules, and with the advent of synthetic biology, novel applications, and tools have been developed for filamentous fungi. Here we summarize approaches aiming for the expression of endogenous or exogenous natural product BGCs, including synthetic transcription factors, assembly of artificial transcription units, gene cluster refactoring, fungal shuttle vectors, and platform strains.

Original languageEnglish
Article number901037
Number of pages22
JournalFrontiers in Bioengineering and Biotechnology
Volume10
DOIs
Publication statusPublished - 15-Jul-2022

Keywords

  • biosynthetic gene cluster
  • fungal platform strains
  • secondary metabilites
  • synthetic biology
  • synthetic transcriptional regulators

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