Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano

Magda Grudniewska, Stijn Mouton, Daniil Simanov, Frank Beltman, Margriet Grelling, Katrien de Mulder, Wibowo Arindrarto, Philipp M. Weissert, Stefan van der Elst, Eugene Berezikov*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)
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Abstract

he regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.

Original languageEnglish
Article number20607
Number of pages23
JournaleLife
Volume5
DOIs
Publication statusPublished - 20-Dec-2016

Keywords

  • PLANARIAN SCHMIDTEA-MEDITERRANEA
  • PLURIPOTENT STEM-CELLS
  • RNA-SEQ DATA
  • GENE-EXPRESSION
  • FLUORESCENT SPERM
  • TRANSPARENT WORM
  • SEXUAL SELECTION
  • REGENERATION
  • FLATWORM
  • GENOME

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