TY - JOUR
T1 - Transcriptomic profiling of induced steatosis in human and mouse precision-cut liver slices
AU - Simon, Eric
AU - Motyka, Maciej
AU - Prins, Grietje H.
AU - Li, Mei
AU - Rust, Werner
AU - Kauschke, Stefan
AU - Viollet, Coralie
AU - Olinga, Peter
AU - Oldenburger, Anouk
N1 - Funding Information:
We thank Holger Klein, Stephan Zahn und Jan-Nygard Jensen for supporting the publication of the study. We thank Karin Fiesel for supporting the RNASeq data preprocessing. We also thank the surgeons of the Hepato-Pancreatico-Biliary Surgery and Liver Transplantation of the University Medical Centre Groningen, among which Vincent E. De Meijer, Koert P. de Jong and Otto B. van Leeuwen, for their help in procuring human liver tissue for this study.
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/5/19
Y1 - 2023/5/19
N2 - There is a high need for predictive human ex vivo models for non-alcoholic fatty liver disease (NAFLD). About a decade ago, precision-cut liver slices (PCLSs) have been established as an ex vivo assay for humans and other organisms. In the present study, we use transcriptomics by RNASeq to profile a new human and mouse PCLSs based assay for steatosis in NAFLD. Steatosis as quantified by an increase of triglycerides after 48 h in culture, is induced by incremental supplementation of sugars (glucose and fructose), insulin, and fatty acids (palmitate, oleate). We mirrored the experimental design for human vs. mouse liver organ derived PCLSs and profiled each organ at eight different nutrient conditions after 24 h and 48 h time in culture. Thus, the provided data allows a comprehensive analysis of the donor, species, time, and nutrient factor specific regulation of gene expression in steatosis, despite the heterogeneity of the human tissue samples. Exemplified this is demonstrated by ranking homologous gene pairs by convergent or divergent expression pattern across nutrient conditions.
AB - There is a high need for predictive human ex vivo models for non-alcoholic fatty liver disease (NAFLD). About a decade ago, precision-cut liver slices (PCLSs) have been established as an ex vivo assay for humans and other organisms. In the present study, we use transcriptomics by RNASeq to profile a new human and mouse PCLSs based assay for steatosis in NAFLD. Steatosis as quantified by an increase of triglycerides after 48 h in culture, is induced by incremental supplementation of sugars (glucose and fructose), insulin, and fatty acids (palmitate, oleate). We mirrored the experimental design for human vs. mouse liver organ derived PCLSs and profiled each organ at eight different nutrient conditions after 24 h and 48 h time in culture. Thus, the provided data allows a comprehensive analysis of the donor, species, time, and nutrient factor specific regulation of gene expression in steatosis, despite the heterogeneity of the human tissue samples. Exemplified this is demonstrated by ranking homologous gene pairs by convergent or divergent expression pattern across nutrient conditions.
UR - https://www.scopus.com/pages/publications/85159672830
U2 - 10.1038/s41597-023-02220-0
DO - 10.1038/s41597-023-02220-0
M3 - Article
C2 - 37208356
AN - SCOPUS:85159672830
SN - 2052-4463
VL - 10
JO - Scientific Data
JF - Scientific Data
IS - 1
M1 - 304
ER -