Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation

Diego Romero; Alejandro Perez-Garcia; Jan-Willem Veening; Antonio de Vicente; Oscar P. Kuipers; Vicente A. de

doi:10.1016/j.mimet.2006.01.005

Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation

Diego Romero, Alejandro Perez-Garcia^*, Jan-Willem Veening, Antonio de Vicente, Oscar P. Kuipers, Vicente A. de

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods. (c) 2006 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	556-559
Number of pages	4
Journal	Journal of microbiological methods
Volume	66
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2006.01.005
Publication status	Published - Sep-2006

Keywords

Bacillus spp.
electrotransformation
genetic manipulation
gram-positive bacteria
ELECTROTRANSFORMATION EFFICIENCY
HIGH OSMOLARITY
PLASMID DNA
REGENERATION
RESISTANCE
BACTERIA

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Cite this

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title = "Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation",

abstract = "A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods. (c) 2006 Elsevier B.V. All rights reserved.",

keywords = "Bacillus spp., electrotransformation, genetic manipulation, gram-positive bacteria, ELECTROTRANSFORMATION EFFICIENCY, HIGH OSMOLARITY, PLASMID DNA, REGENERATION, RESISTANCE, BACTERIA",

author = "Diego Romero and Alejandro Perez-Garcia and Jan-Willem Veening and {de Vicente}, Antonio and Kuipers, {Oscar P.} and de, {Vicente A.}",

year = "2006",

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doi = "10.1016/j.mimet.2006.01.005",

language = "English",

volume = "66",

pages = "556--559",

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AU - Romero, Diego

AU - Perez-Garcia, Alejandro

AU - Veening, Jan-Willem

AU - de Vicente, Antonio

AU - Kuipers, Oscar P.

AU - de, Vicente A.

PY - 2006/9

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AB - A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods. (c) 2006 Elsevier B.V. All rights reserved.

KW - Bacillus spp.

KW - electrotransformation

KW - genetic manipulation

KW - gram-positive bacteria

KW - ELECTROTRANSFORMATION EFFICIENCY

KW - HIGH OSMOLARITY

KW - PLASMID DNA

KW - REGENERATION

KW - RESISTANCE

KW - BACTERIA

U2 - 10.1016/j.mimet.2006.01.005

DO - 10.1016/j.mimet.2006.01.005

M3 - Article

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SP - 556

EP - 559

JO - Journal of microbiological methods

JF - Journal of microbiological methods

SN - 0167-7012

IS - 3

ER -