Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation

Diego Romero, Alejandro Perez-Garcia*, Jan-Willem Veening, Antonio de Vicente, Oscar P. Kuipers, Vicente A. de

*Corresponding author for this work

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Abstract

A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods. (c) 2006 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)556-559
Number of pages4
JournalJournal of microbiological methods
Volume66
Issue number3
DOIs
Publication statusPublished - Sep-2006

Keywords

  • Bacillus spp.
  • electrotransformation
  • genetic manipulation
  • gram-positive bacteria
  • ELECTROTRANSFORMATION EFFICIENCY
  • HIGH OSMOLARITY
  • PLASMID DNA
  • REGENERATION
  • RESISTANCE
  • BACTERIA

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