The amino acid sequence of the sodium ion-dependent citrate transporter CitS of IL pneumoniae contains 12 hydrophobic stretches that could form membrane-spanning segments. A previous analysis of the membrane topology in Escherichia coli using the PhoA gene fusion technique indicated that only nine of these hydrophobic segments span the membrane, while three segments, Vb, VIII and IX were predicted to have a periplasmic location (Van Geest, IM., and Lolkema, J. S. (1996) J. Biol. Chem. 271, 25582-25589), A topology study of C-terminally truncated CitS molecules in dog pancreas microsomes revealed that the protein traverses the endoplasmic reticulum membrane 11 times. In agreement with the PhoA fusion data, segment Vb was predicted to have a periplasmic location, but, in contrast, segments VIII and IX were found to be membrane-spanning (Van Geest, M., Nilsson, I., von Heijne, G., and Lolkema, J, S, (1999) J. Biol, Chem. 274, 2816-2823),
In the present study, using site-directed Cys labeling, the topology of segments VIII and IX in the fall-length CitS protein was determined in the E. coli membrane, Engineered cysteine residues in the loop between the two segments were accessible to a membrane-impermeable thiol reagent exclusively from the cytoplasmic side of the membrane, demonstrating that transmembrane segments (TMSs) VIII and M are both membrane-spanning. It follows that the folding of CitS in the E. cold and endoplasmic reticulum membrane is the same. Cysteine accessibility studies of CitS-PhoA fusion molecules dem; onstrated that in the E. coli membrane segment VIII is exported to the periplasm in the absence of the C-terminal CitS sequences, thus explaining why the PhoA fusions do not correctly predict the topology. An engineered cysteine residue downstream of TMS VIII moved from a periplasmic to a cytoplasmic location when the fusion protein containing TMSs I-VIII was extended with segment IX, Thus, downstream segment M is both essential and sufficient for the insertion of segment VIII of CitS in the E. coli membrane.
- SIGNAL RECOGNITION PARTICLE
- IN-VITRO TRANSLATION
- DEPENDENT CITRATE CARRIER
- PROTEIN TRANSLOCATION