Abstract
Clarithromycin and rifampicin are used for the treatment of Mycobacteria. Pharmacokinetic drug interaction is possibly due to the influence of the two drugs on the liver enzymes. Using a Hypurity Aquastar C18 column (50 mm x 2.1 mm x 5 mu m) for liquid chromatography including a polar end-capped phase for the determination of clarithromycin, rifampicin and their metabolites together in plasma using LC-MS/MS resulted in a substantial carry-over. As a consequence, the throughput of the method is not assured. Using a step-by-step troubleshooting procedure, such carry-over was found originating from column memory effect. With the use of another type of C18 column, the carry-over is eliminated. Due to the absence of carry-over, the analytical concentration ranges are extended and are therefore more appropriate for the analysis of patient samples. The method was re-validated for linearity, reproducibility and dilution integrity. (c) 2013 Elsevier B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 1-4 |
| Number of pages | 4 |
| Journal | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 917 |
| DOIs | |
| Publication status | Published - 15-Feb-2013 |
Keywords
- Rifampicin
- Clarithromycin
- Mycobacteria
- Carry-over
- LC-MS/MS
- CLINICAL PHARMACOKINETICS
- INFECTION
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