Typing of Mycoplasma pneumoniae by PCR-mediated DNA fingerprinting

D Ursi; M Ieven; H van Bever; W Quint; H G Niesters; H Goossens

Typing of Mycoplasma pneumoniae by PCR-mediated DNA fingerprinting

D Ursi, M Ieven, H van Bever, W Quint, H G Niesters, H Goossens

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Abstract

PCR fingerprinting was used to characterize clinical isolates of Mycoplasma pneumoniae. Among 24 strains tested, two types were distinguished. Nineteen strains belonged to type 1, whereas only 5 strains belonged to type 2. The majority of strains isolated since 1991 in Belgium belong to type 1. No variations in fingerprinting patterns were observed within each group, confirming the highly conserved nature of the M. pneumoniae genome.

Original language	English
Pages (from-to)	2873-5
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	32
Issue number	11
Publication status	Published - Nov-1994

Keywords

Bacterial Typing Techniques
Base Sequence
Child
DNA Fingerprinting
Humans
Molecular Sequence Data
Mycoplasma pneumoniae
Nasopharynx
Polymerase Chain Reaction

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J. Clin. Microbiol.-1994-Ursi-2873-5Final publisher's version, 693 KB

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title = "Typing of Mycoplasma pneumoniae by PCR-mediated DNA fingerprinting",

abstract = "PCR fingerprinting was used to characterize clinical isolates of Mycoplasma pneumoniae. Among 24 strains tested, two types were distinguished. Nineteen strains belonged to type 1, whereas only 5 strains belonged to type 2. The majority of strains isolated since 1991 in Belgium belong to type 1. No variations in fingerprinting patterns were observed within each group, confirming the highly conserved nature of the M. pneumoniae genome.",

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AU - Ieven, M

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AU - Quint, W

AU - Niesters, H G

AU - Goossens, H

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AB - PCR fingerprinting was used to characterize clinical isolates of Mycoplasma pneumoniae. Among 24 strains tested, two types were distinguished. Nineteen strains belonged to type 1, whereas only 5 strains belonged to type 2. The majority of strains isolated since 1991 in Belgium belong to type 1. No variations in fingerprinting patterns were observed within each group, confirming the highly conserved nature of the M. pneumoniae genome.

KW - Bacterial Typing Techniques

KW - Base Sequence

KW - Child

KW - DNA Fingerprinting

KW - Humans

KW - Molecular Sequence Data

KW - Mycoplasma pneumoniae

KW - Nasopharynx

KW - Polymerase Chain Reaction

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