Ultrastructure of acantholysis in pemphigus foliaceus re-examined from the current perspective

G. van der Wier*, M. F. Jonkman, H. H. Pas, G. F. H. Diercks

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Background Pemphigus foliaceus (PF) is a chronic cutaneous autoimmune blistering disease that is characterized by superficial blistering of the skin, and according to the current perspective is caused by autoantibodies directed against desmoglein (Dsg) 1.

Objectives To examine early acantholysis in the skin of patients with PF at an ultrastructural level.

Methods Two Nikolsky-negative (N-), five Nikolsky-positive (N+) and two lesional skin biopsies from immunoserologically defined patients with PF were studied by light and electron microscopy.

Results We found no abnormalities in N- PF skin, whereas all the N+ skin biopsies displayed intercellular widening between desmosomes, a decreased number of desmosomes and hypoplastic desmosomes in the lower epidermal layers. Acantholysis was present in two of five N+ biopsies, but only in the upper epidermal layers. The lesional skin biopsies displayed acantholysis in the higher epidermal layers. Hypoplastic desmosomes were partially (pseudo-half-desmosomes) or completely torn off from the opposing cell.

Conclusion We propose the following mechanism for acantholysis in PF: initially PF IgG causes a depletion of nonjunctional Dsg1, leading to intercellular widening between desmosomes starting in the lower layers and spreading upwards. Depletion of nonjunctional Dsg1 impairs the assembly of desmosomes, resulting in hypoplastic desmosomes and a decreased number of desmosomes. In addition, antibodies might promote disassembly of desmosomes. In the upper layers of the epidermis, where Dsg3 is not expressed and cannot compensate for Dsg1 loss, ongoing depletion of Dsg1 will finally result in a total disappearance of desmosomes and subsequent acantholysis.

Original languageEnglish
Pages (from-to)1265-1271
Number of pages7
JournalBRITISH JOURNAL OF DERMATOLOGY
Volume167
Issue number6
DOIs
Publication statusPublished - Dec-2012

Keywords

  • CELL-ADHESION
  • VULGARIS-IGG
  • DESMOGLEIN 3
  • AUTOANTIBODIES
  • DESMOSOMES
  • DEPLETION
  • SKIN
  • KERATINOCYTES
  • LOCALIZATION
  • HYPOTHESIS

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